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Literature summary extracted from

  • Greenberg, J.M.; Thompson, J.F.; Madison, J.T.
    Homoserine kinase and threonine synthase in methionine-overproducing soybean tissue cultures (1988), Plant Cell Rep., 7, 477-480.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.1 L-cysteine strong inhibition at 0.5 mM Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.1 chloroplast not exclusively in stroma Glycine max 9507
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.1 Glycine max
-
 wild type and several methionine-overproducing strains
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.1 cell culture
-
 Glycine max
-
4.2.3.1 cell suspension culture
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.1 additional information
-
 activity with addition of several amino acids to several mutant cell lines Glycine max

Storage Stability

EC Number Storage Stability Organism
4.2.3.1 -80°C, MOPS-buffer, pH 7.5, EDTA, dithioerythritol, 2-benzothiazolethiol, glycerol, polyvinylpyrrolidone, several months, no loss of activity Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.1 O-phospho-L-homoserine + H2O
-
 Glycine max L-threonine + phosphate
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.1 30
-
 enzyme assay at Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.1 7.3
-
 enzyme assay at Glycine max