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Literature summary extracted from

  • Mukhopadhyay, B.; Daniels, L.
    Aerobic purification of N5,N10-methylenetetrahydromethanopterin dehydrogenase, separated from N5,N10-methenyltetrahydromethanopterin cyclohydrolase, from Methanobacterium thermoautotrophicum strain Marburg (1989), Can. J. Microbiol., 35, 499-507.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.98.1 stable in phosphate buffer with or without glycerol or ammonium sulfate under both aerobic and anaerobic conditions Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.98.1 32000
-
x * 32000, SDS-PAGE Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.1 Methanothermobacter thermautotrophicus
-
-
-
1.5.98.1 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.5.98.1 stable under air Methanothermobacter thermautotrophicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.98.1
-
Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.98.1 197
-
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 absolutely dependent on coenzyme F420 Methanothermobacter thermautotrophicus 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 absolutely dependent on coenzyme F420 Methanothermobacter thermautotrophicus Marburg / DSM 2133 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?

Subunits

EC Number Subunits Comment Organism
1.5.98.1 ? x * 32000, SDS-PAGE Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.98.1 coenzyme F420 required Methanothermobacter thermautotrophicus