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Literature summary extracted from

  • Lou, L.; Nakamura, J.; Tsing, S.; Nguyen, B.; Chow, J.; Straub, K.; Chan, H.; Barnett, J.
    High-level production from a baculovirus expression system and biochemical characterization of human GMP synthetase (1995), Protein Expr. Purif., 6, 487-495.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.5.2 synthesis importance of guanine synthesis in immune cell function, GMP synthetase is a potential target for immunosuppressive therapy Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.2 expression in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.2 6-Amino-9-D-psicofuranosylpurine
-
 Homo sapiens
6.3.5.2 Decoyinine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.2 0.0356
-
 XMP native enzyme Homo sapiens
6.3.5.2 0.043
-
 XMP recombinant enzyme Homo sapiens
6.3.5.2 0.131
-
 ATP native enzyme Homo sapiens
6.3.5.2 0.161
-
 ATP recombinant enzyme Homo sapiens
6.3.5.2 0.405
-
 Gln native enzyme Homo sapiens
6.3.5.2 0.44
-
 Gln recombinant enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.2 77360
-
 1 * 77360, density gradient centrifugation and subsequent electrospray MS analysis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.2
-
 Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.2 3.74
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.2 ATP + XMP + NH4+
-
 Homo sapiens AMP + diphosphate + GMP
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.2 monomer 1 * 77360, density gradient centrifugation and subsequent electrospray MS analysis Homo sapiens