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Literature summary extracted from

  • Tokuyasu, K.; Kaneko, S.; Hayashi, K.; Mori, Y.
    Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cells (1999), FEBS Lett., 458, 23-26.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.41
-
 Colletotrichum lindemuthianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.41 0.0405
-
 (N-acetyl-D-glucosamine)5 S1-CDAH Colletotrichum lindemuthianum
3.5.1.41 0.0406
-
 (N-acetyl-D-glucosamine)5 S1-CDA Colletotrichum lindemuthianum
3.5.1.41 0.0416
-
 (N-acetyl-D-glucosamine)5 S12-CDAH Colletotrichum lindemuthianum
3.5.1.41 0.0446
-
 (N-acetyl-D-glucosamine)5 original CDA Colletotrichum lindemuthianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.41 extracellular
-
 Colletotrichum lindemuthianum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.41 chitin + H2O Colletotrichum lindemuthianum
-
 chitosan + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Colletotrichum lindemuthianum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41 recombinant and original CDA enzymes, to homogeneity Colletotrichum lindemuthianum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.41 culture filtrate
-
 Colletotrichum lindemuthianum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 (N-acetyl-D-glucosamine)5
-
 Colletotrichum lindemuthianum (GlcN)4GlcNAc + acetate
-
 ?
3.5.1.41 (N-acetyl-D-glucosamine)5 + H2O
-
 Colletotrichum lindemuthianum (GlcN)4GlcNAc + acetate
-
 ?
3.5.1.41 chitin + H2O
-
 Colletotrichum lindemuthianum chitosan + acetate
-
 ?
3.5.1.41 glycol chitin + H2O
-
 Colletotrichum lindemuthianum chitosan + acetate
-
 ?
3.5.1.41 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Colletotrichum lindemuthianum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 ? S1-CDA: 24700, S1-CDAH: 25700, S12-CDAH: 26800, original CDA: 24300, SDS-PAGE Colletotrichum lindemuthianum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.41 21.5
-
 (N-acetyl-D-glucosamine)5 S12-CDAH Colletotrichum lindemuthianum
3.5.1.41 30.3
-
 (N-acetyl-D-glucosamine)5 S1-CDAH Colletotrichum lindemuthianum
3.5.1.41 47.1
-
 (N-acetyl-D-glucosamine)5 original CDA Colletotrichum lindemuthianum
3.5.1.41 51.6
-
 (N-acetyl-D-glucosamine)5 S1-CDA Colletotrichum lindemuthianum