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Literature summary extracted from
- A pathway of chitosan formation in Mucor rouxii. Enzymatic deacetylation of chitin (1975), Eur. J. Biochem., 55, 71-78.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.41 | 2-mercaptoethanol | - |
Amylomyces rouxii |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.5.1.41 | freezing in presence of ammonium sulfate causes inactivation, dialysis before storage | Amylomyces rouxii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.41 | acetate | - |
Amylomyces rouxii | |
| 3.5.1.41 | Co2+ | - |
Amylomyces rouxii | |
| 3.5.1.41 | EDTA | - |
Amylomyces rouxii | |
| 3.5.1.41 | glycol chitosan | - |
Amylomyces rouxii | |
| 3.5.1.41 | Mn2+ | - |
Amylomyces rouxii | |
| 3.5.1.41 | Na+ | - |
Amylomyces rouxii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.41 | 2.6 | - |
glycol chitin | - |
Amylomyces rouxii |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.41 | extracellular | - |
Amylomyces rouxii | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.41 | Zn2+ | - |
Amylomyces rouxii | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.41 | chitin + H2O | Staphylococcus aureus | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Bacillus subtilis | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Escherichia coli | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Aspergillus niger | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Priestia megaterium | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Enterococcus faecalis | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Bacillus cereus | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Aspergillus oryzae | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Aspergillus parasiticus | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Amylomyces rouxii | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Bacillus subtilis AHU 1031 | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Amylomyces rouxii AHU 6019 | - |
chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | Bacillus subtilis IAM 1069 | - |
chitosan + acetate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.41 | Amylomyces rouxii | - |
AHU 6019 | - |
| 3.5.1.41 | Amylomyces rouxii AHU 6019 | - |
AHU 6019 | - |
| 3.5.1.41 | Aspergillus niger | - |
low enzyme level | - |
| 3.5.1.41 | Aspergillus oryzae | - |
low enzyme level | - |
| 3.5.1.41 | Aspergillus parasiticus | - |
low enzyme level | - |
| 3.5.1.41 | Bacillus cereus | - |
low enzyme level | - |
| 3.5.1.41 | Bacillus subtilis | - |
low enzyme level | - |
| 3.5.1.41 | Bacillus subtilis | - |
AHU 1031 | - |
| 3.5.1.41 | Bacillus subtilis | - |
IAM 1069 | - |
| 3.5.1.41 | Bacillus subtilis AHU 1031 | - |
AHU 1031 | - |
| 3.5.1.41 | Bacillus subtilis IAM 1069 | - |
IAM 1069 | - |
| 3.5.1.41 | Enterococcus faecalis | - |
low enzyme level | - |
| 3.5.1.41 | Escherichia coli | - |
low enzyme level | - |
| 3.5.1.41 | Priestia megaterium | - |
low enzyme level | - |
| 3.5.1.41 | Staphylococcus aureus | - |
low enzyme level | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.41 | 140fold, partial | Amylomyces rouxii |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.41 | culture filtrate | - |
Amylomyces rouxii | - |
| 3.5.1.41 | mycelium | - |
Amylomyces rouxii | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.41 | 17.8 | - |
- |
Amylomyces rouxii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.1.41 | -18°C, stable for several months | Amylomyces rouxii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.41 | chitin + H2O | - |
Staphylococcus aureus | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Bacillus subtilis | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Escherichia coli | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Aspergillus niger | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Priestia megaterium | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Enterococcus faecalis | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Bacillus cereus | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Aspergillus oryzae | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Aspergillus parasiticus | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Amylomyces rouxii | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | colloidal chitin | Amylomyces rouxii | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | powdered chitin, poor | Amylomyces rouxii | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Bacillus subtilis AHU 1031 | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Amylomyces rouxii AHU 6019 | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | colloidal chitin | Amylomyces rouxii AHU 6019 | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | powdered chitin, poor | Amylomyces rouxii AHU 6019 | chitosan + acetate | - |
? | |
| 3.5.1.41 | chitin + H2O | - |
Bacillus subtilis IAM 1069 | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Staphylococcus aureus | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Bacillus subtilis | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Escherichia coli | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Aspergillus niger | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Priestia megaterium | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Enterococcus faecalis | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Bacillus cereus | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Aspergillus oryzae | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Aspergillus parasiticus | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Amylomyces rouxii | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Bacillus subtilis AHU 1031 | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Amylomyces rouxii AHU 6019 | chitosan + acetate | - |
? | |
| 3.5.1.41 | glycol chitin + H2O | - |
Bacillus subtilis IAM 1069 | chitosan + acetate | - |
? | |
| 3.5.1.41 | N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Amylomyces rouxii | ? | - |
? | |
| 3.5.1.41 | N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Amylomyces rouxii | ? | - |
? | |
| 3.5.1.41 | N,N',N''-triacetylchitotriose + H2O | poor | Amylomyces rouxii | ? | - |
? | |
| 3.5.1.41 | N,N',N''-triacetylchitotriose + H2O | poor | Amylomyces rouxii AHU 6019 | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.41 | 5.5 | - |
- |
Amylomyces rouxii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.41 | additional information | - |
- |
Amylomyces rouxii |
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Release 2023.1 (January 2023)
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