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Literature summary extracted from

  • Sawada, H.; Yokosawa, H.; Ishii, S.
    Purification and characterization of two types of trypsin-like enzymes from sperm of the ascidian (Prochordata) Halocynthia roretzi. Evidence for the presence of spermosin, a novel acrosin-like enzyme (1984), J. Biol. Chem., 259, 2900-2904.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.99 bovine serum albumin stabilizes Halocynthia roretzi
3.4.21.99 non-ionic detergents such as Brij 35, Triton X-100, Tween 80 at concentrations between 0.005% and 0.1% stabilize Halocynthia roretzi
3.4.21.99 stable in presence of contaminating proteins, very labile in their absence Halocynthia roretzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.99 antipain
-
Halocynthia roretzi
3.4.21.99 benzamidine
-
Halocynthia roretzi
3.4.21.99 diisopropyl fluorophosphate
-
Halocynthia roretzi
3.4.21.99 EGTA
-
Halocynthia roretzi
3.4.21.99 Gly-Val-Arg-CH2Cl
-
Halocynthia roretzi
3.4.21.99 HgCl2
-
Halocynthia roretzi
3.4.21.99 N-acetyl-Leu-Leu-arginal
-
Halocynthia roretzi
3.4.21.99 ovomucoid
-
Halocynthia roretzi
3.4.21.99 p-aminobenzamidine
-
Halocynthia roretzi
3.4.21.99 phenylmethanesulfonyl fluoride
-
Halocynthia roretzi
3.4.21.99 Soybean trypsin inhibitor
-
Halocynthia roretzi
3.4.21.99 ZnCl2
-
Halocynthia roretzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.99 0.145
-
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide
-
Halocynthia roretzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.99 Ca2+ maximum activity with 1-5 mM Ca2+, the presence of Ca2+ is not indispensable Halocynthia roretzi
3.4.21.99 additional information not strongly influenced by changing salt concentrations except Ca2+ Halocynthia roretzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.99 27000
-
gel filtration, SDS-PAGE Halocynthia roretzi

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.99 Halocynthia roretzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.99
-
Halocynthia roretzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.99 spermatozoon
-
Halocynthia roretzi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.99 2
-
-
Halocynthia roretzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.99 tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O
-
Halocynthia roretzi tert-butyloxycarbonyl-Val-Pro + Arg-4-methylcoumarin-7-amide
-
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Subunits

EC Number Subunits Comment Organism
3.4.21.99 monomer 1 * 27000, SDS-PAGE Halocynthia roretzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.99 8.5 9
-
Halocynthia roretzi