Literature summary extracted from

  • Robinson, M.B.; Blakely, R.D.; Couto, R.; Coyle, J.T.
    Hydrolysis of the brain dipeptide N-acetyl-L-aspartyl-L-glutamate (1987), J. Biol. Chem., 262, 14498-14506.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.21 1,10-phenanthroline 1 mM, 90% inhibition Rattus norvegicus
3.4.17.21 2-oxoglutarate 0.1 mM, 46% inhibition Rattus norvegicus
3.4.17.21 Ala-Glu 0.0061 mM, 50% inhibition Rattus norvegicus
3.4.17.21 Asp-Ala 0.1 mM, 26% inhibition Rattus norvegicus
3.4.17.21 aspartate 0.1 mM, 42% inhibition Rattus norvegicus
3.4.17.21 Asp-Asp 0.1 mM, 42% inhibition Rattus norvegicus
3.4.17.21 Asp-Glu 0.0024 mM, 50% inhibition Rattus norvegicus
3.4.17.21 Asp-Phe 0.1 mM, 22% inhibition Rattus norvegicus
3.4.17.21 bestatin 0.29 mM, 79% inhibition Rattus norvegicus
3.4.17.21 Cd2+
-
Rattus norvegicus
3.4.17.21 Cu2+
-
Rattus norvegicus
3.4.17.21 cysteate 0.1 mM, 40% inhibition Rattus norvegicus
3.4.17.21 cysteine sulfinate 0.1 mM, 42% inhibition Rattus norvegicus
3.4.17.21 D-Glu-D-Glu 0.1 mM, 50% inhibition Rattus norvegicus
3.4.17.21 dithiothreitol 1 mM, 94.8% inhibition Rattus norvegicus
3.4.17.21 EDTA 1 mM, 96.3% inhibition Rattus norvegicus
3.4.17.21 EGTA 1 mM, 92% inhibition Rattus norvegicus
3.4.17.21 Fe3+
-
Rattus norvegicus
3.4.17.21 gamma-Glu-Glu 0.0095 mM, 50% inhibition Rattus norvegicus
3.4.17.21 Glu-Asp 0.1 mM, 27% inhibition Rattus norvegicus
3.4.17.21 Glu-Glu 0.00075 mM, 50% inhibition Rattus norvegicus
3.4.17.21 Glu-Glu-Glu 0.062 mM, 50% inhibition Rattus norvegicus
3.4.17.21 glutathione i.e. gamma-Glu-Cys-Gly, 0.019 mM, 50% inhibition Rattus norvegicus
3.4.17.21 Gly-Glu 0.008 mM, 50% inhibition Rattus norvegicus
3.4.17.21 Gly-Gly-Glu 0.00098 mM, 50% inhibition Rattus norvegicus
3.4.17.21 Ibotenate 0.1 mM, 45% inhibition Rattus norvegicus
3.4.17.21 iodoacetate 1 mM, 38% inhibition Rattus norvegicus
3.4.17.21 L-2-Amino-4-phosphonobutanoate 0.1 mM, 37% inhibition Rattus norvegicus
3.4.17.21 L-Glu 0.032 mM, 50% inhibition Rattus norvegicus
3.4.17.21 additional information not inhibitory are pepstatin, iodoacetamide, amastatin Rattus norvegicus
3.4.17.21 N-Acetyl-Asp-Gln 0.1 mM, 49% inhibition Rattus norvegicus
3.4.17.21 N-Acetyl-Asp-Glu-Asp 0.1 mM, 49% inhibition Rattus norvegicus
3.4.17.21 N-Acetyl-Asp-Ser-Gly 0.1 mM, 37% inhibition Rattus norvegicus
3.4.17.21 N-Acetyl-Glu-Asp 0.036 mM, 50% inhibition Rattus norvegicus
3.4.17.21 N-Acetyl-Glu-Glu 0.00031 mM, 50% inhibition Rattus norvegicus
3.4.17.21 N-acetyl-L-Asp 0.1 mM, 38% inhibition Rattus norvegicus
3.4.17.21 N-Acetyl-L-Asp-L-Glu 0.00054 mM, 50% inhibition Rattus norvegicus
3.4.17.21 N-acetyl-L-Glu 0.058 mM, 50% inhibition Rattus norvegicus
3.4.17.21 Phe-Glu 0.077 mM, 50% inhibition Rattus norvegicus
3.4.17.21 PO43-
-
Rattus norvegicus
3.4.17.21 puromycin 0.5 mM, 55% inhibition Rattus norvegicus
3.4.17.21 pyroglutamate 0.1 mM, 27% inhibition Rattus norvegicus
3.4.17.21 quinolinate 0.1 mM, 41% inhibition Rattus norvegicus
3.4.17.21 Quisqualic acid 0.00048 mM, 50% inhibition Rattus norvegicus
3.4.17.21 Serine O-sulfate 0.042 mM, 50% inhibition Rattus norvegicus
3.4.17.21 SO42-
-
Rattus norvegicus
3.4.17.21 Tyr-Glu 0.1 mM, 45% inhibition Rattus norvegicus
3.4.17.21 Val-Glu-Glu 0.036 mM, 50% inhibition Rattus norvegicus
3.4.17.21 Zn2+
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.21 540
-
N-Acetyl-L-Asp-L-Glu
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.17.21 membrane
-
Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.21 Mg2+ activation Rattus norvegicus
3.4.17.21 Mn2+ activation Rattus norvegicus
3.4.17.21 Ca2+ activation Rattus norvegicus
3.4.17.21 Co2+ activation Rattus norvegicus
3.4.17.21 Sr2+ activation Rattus norvegicus
3.4.17.21 Cl- 2 mM, activation Rattus norvegicus
3.4.17.21 Br- 2 mM, activation Rattus norvegicus
3.4.17.21 I- 2 mM, activation Rattus norvegicus
3.4.17.21 NO3- 2 mM , activation Rattus norvegicus
3.4.17.21 F- 100 mM, 20% of the activity compared to Cl- Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.21 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.21 brain forebrain Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.21 additional information
-
assay method Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.21 N-Acetyl-L-Asp-L-Glu + H2O
-
Rattus norvegicus N-Acetyl-L-Asp + L-Glu
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.21 37
-
-
Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.17.21 25 80 more than 50% of maximal activity in this range Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.21 7
-
-
Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.17.21 6 7.4 more than 50% of maximal activity in this range Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.17.21 0.1
-
D-Glu-D-Glu
-
Rattus norvegicus
3.4.17.21 0.062
-
Glu-Glu-Glu
-
Rattus norvegicus
3.4.17.21 0.058
-
N-acetyl-L-Glu
-
Rattus norvegicus
3.4.17.21 0.042
-
Serine O-sulfate
-
Rattus norvegicus
3.4.17.21 0.036
-
N-Acetyl-Glu-Asp
-
Rattus norvegicus
3.4.17.21 0.036
-
Val-Glu-Glu
-
Rattus norvegicus
3.4.17.21 0.00031
-
N-Acetyl-Glu-Glu
-
Rattus norvegicus
3.4.17.21 0.00054
-
N-Acetyl-L-Asp-L-Glu
-
Rattus norvegicus
3.4.17.21 0.077
-
Phe-Glu
-
Rattus norvegicus
3.4.17.21 0.061
-
Ala-Glu
-
Rattus norvegicus
3.4.17.21 0.019
-
glutathione i.e. gamma-Glu-Cys-Gly Rattus norvegicus
3.4.17.21 0.00075
-
Glu-Glu
-
Rattus norvegicus
3.4.17.21 0.095
-
gamma-Glu-Glu
-
Rattus norvegicus
3.4.17.21 0.0024
-
Asp-Glu
-
Rattus norvegicus
3.4.17.21 0.008
-
Gly-Glu
-
Rattus norvegicus
3.4.17.21 0.00098
-
Gly-Gly-Glu
-
Rattus norvegicus