BRENDA - Enzyme Database show

Cloning and nucleotide sequence of the cyclic AMP receptor protein-regulated Salmonella typhimurium pepE gene and crystallization of its product, an alpha-aspartyl dipeptidase

Conlin, C.A.; Hakensson, K.; Liljas, A.; Miller, C.G.; J. Bacteriol. 176, 166-172 (1994)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.4.13.21
-
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.4.13.21
-
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.13.21
beta-Alanine
-
Salmonella enterica subsp. enterica serovar Typhimurium
3.4.13.21
L-Asp
-
Salmonella enterica subsp. enterica serovar Typhimurium
3.4.13.21
additional information
not inhibited by EDTA, dipicolinic acid, DL-2-amino-2-methylpropanoate
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.13.21
4.3
-
L-Asp-4-nitroanilide
-
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.13.21
additional information
the enzyme does not contain zinc or any other metals
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.13.21
24770
-
calculation from sequence of cDNA
Salmonella enterica subsp. enterica serovar Typhimurium
3.4.13.21
27000
-
SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.13.21
Salmonella enterica subsp. enterica serovar Typhimurium
-
GenBank accession number U01246
-
Purification (Commentary)
EC Number
Commentary
Organism
3.4.13.21
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.13.21
L-Asp-4-nitroanilide + H2O
-
137205
Salmonella enterica subsp. enterica serovar Typhimurium
L-Asp + 4-nitroaniline
-
137205
Salmonella enterica subsp. enterica serovar Typhimurium
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.13.21
monomer
1 * 27000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.13.21
39
-
L-Asp-4-nitroanilide
-
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.13.21
7
7.5
-
Salmonella enterica subsp. enterica serovar Typhimurium
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.13.21
6.5
8
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.13.21
-
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.4.13.21
-
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.13.21
beta-Alanine
-
Salmonella enterica subsp. enterica serovar Typhimurium
3.4.13.21
L-Asp
-
Salmonella enterica subsp. enterica serovar Typhimurium
3.4.13.21
additional information
not inhibited by EDTA, dipicolinic acid, DL-2-amino-2-methylpropanoate
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.13.21
4.3
-
L-Asp-4-nitroanilide
-
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.13.21
additional information
the enzyme does not contain zinc or any other metals
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.13.21
24770
-
calculation from sequence of cDNA
Salmonella enterica subsp. enterica serovar Typhimurium
3.4.13.21
27000
-
SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.13.21
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.13.21
L-Asp-4-nitroanilide + H2O
-
137205
Salmonella enterica subsp. enterica serovar Typhimurium
L-Asp + 4-nitroaniline
-
137205
Salmonella enterica subsp. enterica serovar Typhimurium
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.13.21
monomer
1 * 27000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.13.21
39
-
L-Asp-4-nitroanilide
-
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.13.21
7
7.5
-
Salmonella enterica subsp. enterica serovar Typhimurium
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.13.21
6.5
8
-
Salmonella enterica subsp. enterica serovar Typhimurium