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Literature summary extracted from

  • Hayman, S.; Patterson, E.K.
    Purification and properties of a mouse ascites tumor dipeptidase, a metalloenzyme (1971), J. Biol. Chem., 246, 660-669.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.18 1,10-phenanthroline 1,7-phenanthroline has no effect Mus musculus
3.4.13.18 Co2+ hydrolysis of the relative poor substrates, Pro-Gly and Gly-Gly is activated, whereas that of Ala-Gly is inhibited Mus musculus
3.4.13.18 Mn2+ hydrolysis of the relative poor substrates, Pro-Gly and Gly-Gly is activated, whereas that of Ala-Gly is inhibited Mus musculus
3.4.13.18 additional information inhibition by high substrate concentrations is observed in cases where the Km is low Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.18 0.4
-
Ala-Leu
-
Mus musculus
3.4.13.18 22
-
Gly-L-Ala
-
Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.18 Co2+ hydrolysis of the relative poor substrates, Pro-Gly and Gly-Gly is activated, whereas that of Ala-Gly is inhibited Mus musculus
3.4.13.18 Mn2+ hydrolysis of the relative poor substrates, Pro-Gly and Gly-Gly is activated, whereas that of Ala-Gly is inhibited Mus musculus
3.4.13.18 Zn2+ 0.9 gatom of zinc per mol of enzyme Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.18 85000
-
gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.18 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.18
-
Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.13.18 ascites tumor cell
-
Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.18 Ala-Ile + H2O best substrate for the tumor enzyme Mus musculus Ala + Ile
-
?
3.4.13.18 Ala-Leu + H2O
-
Mus musculus Ala + Leu
-
?
3.4.13.18 Gly-L-Ala + H2O
-
Mus musculus Gly + L-Ala
-
?
3.4.13.18 L-Ala-Gly + H2O
-
Mus musculus L-Ala + Gly
-
?
3.4.13.18 additional information peptides with small NH2-terminal and bulky nonpolar COOH-terminal R groups are preferred Mus musculus ?
-
?
3.4.13.18 additional information only unsubstituted L-alpha-dipeptides Mus musculus ?
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.13.18 43.3
-
Ala-Gly
-
Mus musculus
3.4.13.18 33300
-
Ala-Ile
-
Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.18 8.3
-
-
Mus musculus