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Literature summary extracted from

  • Patterson, E.K.
    A dipeptidase from Escherichia coli B (1976), Methods Enzymol., 45, 377-386.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.13.18 stable to 18 h dialysis, at 10°C against 0.02 M phosphate buffer, pH 7.3-8.3 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.18 2-mercaptoethanol
-
Escherichia coli
3.4.13.18 Co2+ when substrates are cleaved rapidly by dipeptidase, addition of Co2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect Escherichia coli
3.4.13.18 cysteine
-
Escherichia coli
3.4.13.18 D-Forms of dipeptides
-
Escherichia coli
3.4.13.18 DTT
-
Escherichia coli
3.4.13.18 iodoacetate
-
Escherichia coli
3.4.13.18 Mn2+ when substrates are cleaved rapidly by dipeptidase, addition of Mn2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.18 Co2+ when substrates are cleaved rapidly by dipeptidase, addition of Co2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect, activation is not accompanied by change in Km Escherichia coli
3.4.13.18 Co2+ enzyme contains two binding sites Escherichia coli
3.4.13.18 Mn2+ when substrates are cleaved rapidly by dipeptidase, addition of Mn2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect, activation is accompanied by an increase in Km Escherichia coli
3.4.13.18 Mn2+ two binding sites, at least one of which is distinct from a Co2+ site Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.18 53000
-
2 * 53000, SDS-PAGE Escherichia coli
3.4.13.18 100000
-
gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.18 Escherichia coli
-
-
-
3.4.13.18 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.18 strain B Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.18 760
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.4.13.18 -30°C, pH 7.5-8.5, 0.25 M sucrose, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.18 L-Ala-Gly + H2O
-
Escherichia coli L-Ala + Gly
-
?
3.4.13.18 L-Ala-Gly + H2O
-
Escherichia coli B / ATCC 11303 L-Ala + Gly
-
?
3.4.13.18 additional information
-
Escherichia coli ?
-
?
3.4.13.18 additional information
-
Escherichia coli B / ATCC 11303 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.13.18 dimer 2 * 53000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.13.18 40
-
assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.13.18 30
-
30 min: stable, 24 h: 40% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.18 8
-
-
Escherichia coli