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Literature summary extracted from
- Expression, purification, and characterization of recombinant S-adenosylhomocysteine hydrolase from the thermophilic archaeon Sulfolobus solfactorius (2000), Protein Expr. Purif., 18, 27-35.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | medicine | antiviral drug design | Saccharolobus solfataricus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | into Escherichia coli | Saccharolobus solfataricus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.13.2.1 | unusual stability in protein denaturants and detergents, complete recombinant enzyme | Saccharolobus solfataricus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | 0.014 | - |
S-adenosyl-L-homocysteine | complete recombinant enzyme | Saccharolobus solfataricus |  |
| 3.13.2.1 | 0.016 | - |
S-adenosyl-L-homocysteine | truncated recombinant enzyme | Saccharolobus solfataricus | |
| 3.13.2.1 | 0.04 | - |
adenosine | complete recombinant enzyme | Saccharolobus solfataricus | |
| 3.13.2.1 | 0.042 | - |
adenosine | truncated recombinant enzyme | Saccharolobus solfataricus | |
| 3.13.2.1 | 0.148 | - |
L-homocysteine | truncated recombinant enzyme | Saccharolobus solfataricus | |
| 3.13.2.1 | 0.152 | - |
L-homocysteine | complete recombinant enzyme | Saccharolobus solfataricus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 190000 | - |
recombinant enzymes, gel filtration | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Saccharolobus solfataricus | - |
adenosine + L-homocysteine | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | truncated recombinant enzyme: 71.7fold, complete recombinant enzyme: to homogeneity | Saccharolobus solfataricus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine | reaction equilibrium is in vitro strongly in direction of synthesis of S-adenosyl-L-homocysteine | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 0.163 | - |
complete recombinant enzyme | Saccharolobus solfataricus |
| 3.13.2.1 | 0.165 | - |
truncated recombinant enzyme, wild-type | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Saccharolobus solfataricus | adenosine + L-homocysteine | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | tetramer | homotetramer, recombinant enzymes, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 75 | - |
recombinant enzymes | Saccharolobus solfataricus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | additional information | - |
truncated recombinant enzyme | Saccharolobus solfataricus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 88 | - |
melting temperature, truncated recombinant enzyme | Saccharolobus solfataricus |
| 3.13.2.1 | 95 | - |
melting temperature, complete recombinant enzyme | Saccharolobus solfataricus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 7.5 | - |
complete recombinant enzyme | Saccharolobus solfataricus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.2.1 | NAD+ | - |
Saccharolobus solfataricus | |
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