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Literature summary extracted from

  • Poulton, J.E.; Butt, V.S.
    Purification and properties of S-adenosyl-L-homocysteine hydrolase from leaves of spinach beet (1976), Arch. Biochem. Biophys., 172, 135-142.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.13.2.1 adenosine deaminase
-
 Beta vulgaris

Application

EC Number Application Comment Organism
3.13.2.1 analysis study of methylation reactions Beta vulgaris

General Stability

EC Number General Stability Organism
3.13.2.1 unstable in solutions containing low level of protein, stabilization with bovine serum albumin Beta vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.2.1 adenine
-
 Beta vulgaris
3.13.2.1 DL-homocysteine
-
 Beta vulgaris
3.13.2.1 S-adenosyl-L-methionine
-
 Beta vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.1 0.0128
-
 adenosine
-
 Beta vulgaris
3.13.2.1 0.041
-
 S-adenosyl-L-homocysteine
-
 Beta vulgaris
3.13.2.1 1.2
-
 L-homocysteine
-
 Beta vulgaris
3.13.2.1 2.2
-
 DL-homocysteine
-
 Beta vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.2.1 S-adenosyl-L-homocysteine + H2O Beta vulgaris
-
 adenosine + L-homocysteine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Beta vulgaris
-
 L. ssp. vulgaris
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.2.1 100fold Beta vulgaris

Reaction

EC Number Reaction Comment Organism Reaction ID
3.13.2.1 S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine reaction equilibrium is in vitro strongly in direction of synthesis of S-adenosyl-L-homocysteine Beta vulgaris
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.13.2.1 leaf
-
 Beta vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.2.1 2.84
-
-
 Beta vulgaris

Storage Stability

EC Number Storage Stability Organism
3.13.2.1 -20°C, with 5 mM beta-mercaptoethanol and 2 mg/ml bovine serum albumin, stable for several months Beta vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.1 DL-homocysteine + 3-deazaadenosine
-
 Beta vulgaris ?
-
 r
3.13.2.1 DL-homocysteine + adenosine
-
 Beta vulgaris S-adenosyl-DL-homocysteine
-
 r
3.13.2.1 S-adenosyl-L-homocysteine + H2O
-
 Beta vulgaris adenosine + L-homocysteine
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.2.1 8.5
-
 synthesis and hydrolysis of S-adenosyl-L-homocysteine Beta vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.13.2.1 additional information
-
-
 Beta vulgaris
3.13.2.1 6.5 10 more than 50% of activity Beta vulgaris