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Literature summary extracted from

  • Guranowski, A.; Pawelkiewicz, J.
    Adenosylhomocysteinase from yellow lupin seeds. Purification and properties (1977), Eur. J. Biochem., 80, 517-523.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.13.2.1 adenosine deaminase
-
Lupinus luteus
3.13.2.1 adenosine nucleosidase
-
Lupinus luteus

General Stability

EC Number General Stability Organism
3.13.2.1 purified enzyme tolerates repeated freeze-thawing Lupinus luteus
3.13.2.1 tendency of purified enzyme to form multimers Lupinus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.2.1 N-ethylmaleimide
-
Lupinus luteus
3.13.2.1 p-hydroxymercuribenzoate
-
Lupinus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.1 0.0023
-
adenosine
-
Lupinus luteus
3.13.2.1 0.012
-
S-adenosyl-L-homocysteine
-
Lupinus luteus
3.13.2.1 4.6
-
DL-homocysteine
-
Lupinus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.2.1 55000
-
SDS-PAGE Lupinus luteus
3.13.2.1 110000
-
gel filtration Lupinus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.2.1 S-adenosyl-L-homocysteine + H2O Lupinus luteus
-
adenosine + L-homocysteine
-
r

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Lupinus luteus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.13.2.1 2-mercaptoethanol essential for enzymatic activity, in absence 50% loss of activity Lupinus luteus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.2.1 1193.5fold to homogeneity Lupinus luteus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.13.2.1 S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine reaction equilibrium is in vitro strongly in direction of synthesis of S-adenosyl-L-homocysteine Lupinus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.13.2.1 seed
-
Lupinus luteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.2.1 3.7 4.7
-
Lupinus luteus

Storage Stability

EC Number Storage Stability Organism
3.13.2.1 -10°C, loss of 50% of activity after 1 month Lupinus luteus
3.13.2.1 -20°C, stable for at least 2 months Lupinus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.1 7-deaza-8-azaadenosine + homocysteine formycin A Lupinus luteus ?
-
r
3.13.2.1 adenosine N1-oxide + homocysteine
-
Lupinus luteus ?
-
r
3.13.2.1 carboxylic analogue of adenosine
-
Lupinus luteus ?
-
r
3.13.2.1 DL-homocysteine + adenosine
-
Lupinus luteus S-adenosyl-DL-homocysteine
-
r
3.13.2.1 nebularine + homocysteine
-
Lupinus luteus ?
-
r
3.13.2.1 S-adenosyl-L-homocysteine + H2O
-
Lupinus luteus adenosine + L-homocysteine
-
r

Subunits

EC Number Subunits Comment Organism
3.13.2.1 dimer 2 * 55000, pore gradient gel electrophoresis Lupinus luteus
3.13.2.1 monomer 1 * 55000, pore gradient gel electrophoresis, only dimeric form is enzymatically active Lupinus luteus
3.13.2.1 tetramer 4 * 55000, pore gradient gel electrophoresis Lupinus luteus
3.13.2.1 trimer 3 * 55000, pore gradient gel electrophoresis Lupinus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.2.1 8.5 9
-
Lupinus luteus