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Literature summary extracted from

  • Hershfield, M.S.; Aiyar, V.N.; Premakumar, R.; Small, W.C.
    S-Adenosylhomocysteine hydrolase from human placenta. Affinity purification and characterization (1985), Biochem. J., 230, 43-52.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.2.1 4',5'-dehydroadenosine
-
 Homo sapiens
3.13.2.1 adenine arabinoside
-
 Homo sapiens
3.13.2.1 adenosine
-
 Homo sapiens
3.13.2.1 dithiothreitol together with adenosine Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.1 0.0009
-
 adenosine
-
 Homo sapiens
3.13.2.1 0.0076
-
 S-adenosyl-L-homocysteine
-
 Homo sapiens
3.13.2.1 0.11
-
 4',5'-dehydroadenosine
-
 Homo sapiens
3.13.2.1 0.2
-
 L-homocysteine
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.2.1 49000
-
 4 * 47000-48000, SDS-PAGE, 4 * 49000, amino acid determination Homo sapiens
3.13.2.1 189000
-
 analytical ultrafiltration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.2.1 S-adenosyl-L-homocysteine + H2O Homo sapiens
-
 adenosine + L-homocysteine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.2.1 2200fold to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.13.2.1 CCRF-CEM cell
-
 Homo sapiens
-
3.13.2.1 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.2.1 additional information
-
 specific activity is correlated with enzyme-bound NAD+/NADH ratio Homo sapiens
3.13.2.1 4.4
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.13.2.1 -70°C, 1-2 mg enzyme per ml, loss of less than 10% of activity over 6 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.1 4',5'-dehydroadenosine + H2O favors formation of 4',5'-dehydroadenosine, precursor reaction Homo sapiens adenosine
-
 r
3.13.2.1 S-adenosyl-L-homocysteine + H2O
-
 Homo sapiens adenosine + L-homocysteine
-
 r

Subunits

EC Number Subunits Comment Organism
3.13.2.1 tetramer 4 * 47000-48000, SDS-PAGE, 4 * 49000, amino acid determination Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.13.2.1 37
-
 97% of activity is lost Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
3.13.2.1 NAD+
-
 Homo sapiens