BRENDA - Enzyme Database show

Topography, purification and characterization of thyroidal NAD+ glycohydrolase

De Wolf, M.J.S.; van Dessel, G.A.F.; Lagrou, A.R.; Hilderson, H.J.J.; Dierick, W.S.H.; Biochem. J. 226, 415-427 (1985)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.2.2.5
chromatin NADase is sensitiv to Dnase
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.5
ADPribose
-
Bos taurus
3.2.2.5
nicotinamide
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.5
0.4
-
NAD+
-
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.2.5
12000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.5
NAD+ + H2O
Bos taurus
-
ADPribose + nicotinamide
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.2.5
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.2.5
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.5
thyroid gland
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.2.5
77.6
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.5
additional information
NADP is not cleaved by chromatin NADase
137079
Bos taurus
?
-
-
-
-
3.2.2.5
NAD+ + H2O
-
137079
Bos taurus
ADPribose + nicotinamide
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.5
45
-
1 min, total inactivation of chromatin NADase
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.5
7.2
-
-
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.2.5
chromatin NADase is sensitiv to Dnase
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.5
ADPribose
-
Bos taurus
3.2.2.5
nicotinamide
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.5
0.4
-
NAD+
-
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.2.5
12000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.5
NAD+ + H2O
Bos taurus
-
ADPribose + nicotinamide
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.5
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.5
thyroid gland
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.2.5
77.6
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.5
additional information
NADP is not cleaved by chromatin NADase
137079
Bos taurus
?
-
-
-
-
3.2.2.5
NAD+ + H2O
-
137079
Bos taurus
ADPribose + nicotinamide
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.5
45
-
1 min, total inactivation of chromatin NADase
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.5
7.2
-
-
Bos taurus