BRENDA - Enzyme Database show

Nicotinamide adenine dinucleotide glycohydrolases from Ehrlich ascites tumor cell nuclei: isolation, partial purification and properties

Green, S.; Dobrjansky, A.; Biochemistry 11, 4108-4113 (1972)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.2.2.5
chromatin NADase is sensitiv to Dnase
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.5
additional information
nuclear membrane NADase is insensitiv to 1 M NaCl
Mus musculus
3.2.2.5
NaCl
0.5 M or more, 65% loss in activity, irreversible, DNA associated NADase
Mus musculus
3.2.2.5
nicotinamide
0.33 mM, 50% inhibition
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.2.5
nucleus
-
Mus musculus
5634
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.5
NAD+ + H2O
Mus musculus
-
ADPribose + nicotinamide
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.2.5
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.2.5
partial
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.5
Ehrlich ascites carcinoma cell
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.5
NAD+ + H2O
-
137075
Mus musculus
ADPribose + nicotinamide
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.5
45
-
2 min, 100% inactivation, DNA associated NADase; 5 min, stable, nuclear membrane NADase
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.5
6.3
7
nuclear membrane
Mus musculus
3.2.2.5
6.6
7.1
DNA associated NADase
Mus musculus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.2.5
0.33
-
nicotinamide
-
Mus musculus
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.2.5
chromatin NADase is sensitiv to Dnase
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.5
additional information
nuclear membrane NADase is insensitiv to 1 M NaCl
Mus musculus
3.2.2.5
NaCl
0.5 M or more, 65% loss in activity, irreversible, DNA associated NADase
Mus musculus
3.2.2.5
nicotinamide
0.33 mM, 50% inhibition
Mus musculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.2.5
0.33
-
nicotinamide
-
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.2.5
nucleus
-
Mus musculus
5634
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.5
NAD+ + H2O
Mus musculus
-
ADPribose + nicotinamide
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.5
partial
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.5
Ehrlich ascites carcinoma cell
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.5
NAD+ + H2O
-
137075
Mus musculus
ADPribose + nicotinamide
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.5
45
-
2 min, 100% inactivation, DNA associated NADase; 5 min, stable, nuclear membrane NADase
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.5
6.3
7
nuclear membrane
Mus musculus
3.2.2.5
6.6
7.1
DNA associated NADase
Mus musculus