BRENDA - Enzyme Database show

NAD glycohydrolases from rat liver nuclei

Okayama, H.; Ueda, K.; Hayaishi, O.; Methods Enzymol. 66, 151-154 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.5
Alpha-NAD+
5 mM, 70% inhibition
Rattus norvegicus
3.2.2.5
deamino-NAD+
5 mM, almost complete inhibition
Rattus norvegicus
3.2.2.5
NADP+
5 mM, almost complete inhibition
Rattus norvegicus
3.2.2.5
nicotinamide
50 mM, 33% inhibition
Rattus norvegicus
3.2.2.5
NMN
5 mM, 70% inhibition
Rattus norvegicus
3.2.2.5
thioNAD+
5 mM, almost complete inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.5
0.03
-
NAD+
chromatin
Rattus norvegicus
3.2.2.5
0.06
-
NAD+
nuclear envelope
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.2.5
microsome
-
Rattus norvegicus
-
-
3.2.2.5
nucleus
chromatin and nuclear envelope
Rattus norvegicus
5634
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.5
NAD+ + H2O
Rattus norvegicus
-
ADPribose + nicotinamide
-
-
?
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.2.2.5
dithiothreitol
1 mM, slight inactivation
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.2.5
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.2.5
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.5
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.5
3-acetylpyridine adenine dinucleotide + H2O
-
137054
Rattus norvegicus
?
-
-
-
?
3.2.2.5
NAD+ + H2O
-
137054
Rattus norvegicus
ADPribose + nicotinamide
-
-
-
?
3.2.2.5
NADP+ + H2O
-
137054
Rattus norvegicus
phospho-ADPribose + nicotinamide
-
-
-
?
3.2.2.5
NMN + H2O
-
137054
Rattus norvegicus
phosphoribose + nicotinamide
-
-
-
?
3.2.2.5
thioNAD+ + H2O
-
137054
Rattus norvegicus
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.5
6.5
7
chromatin
Rattus norvegicus
3.2.2.5
6.5
-
nuclear envelope
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.2.5
4.5
-
5 min, 60°C, retains of 55% of activity
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.5
Alpha-NAD+
5 mM, 70% inhibition
Rattus norvegicus
3.2.2.5
deamino-NAD+
5 mM, almost complete inhibition
Rattus norvegicus
3.2.2.5
NADP+
5 mM, almost complete inhibition
Rattus norvegicus
3.2.2.5
nicotinamide
50 mM, 33% inhibition
Rattus norvegicus
3.2.2.5
NMN
5 mM, 70% inhibition
Rattus norvegicus
3.2.2.5
thioNAD+
5 mM, almost complete inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.5
0.03
-
NAD+
chromatin
Rattus norvegicus
3.2.2.5
0.06
-
NAD+
nuclear envelope
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.2.5
microsome
-
Rattus norvegicus
-
-
3.2.2.5
nucleus
chromatin and nuclear envelope
Rattus norvegicus
5634
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.5
NAD+ + H2O
Rattus norvegicus
-
ADPribose + nicotinamide
-
-
?
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.2.2.5
dithiothreitol
1 mM, slight inactivation
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.5
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.5
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.5
3-acetylpyridine adenine dinucleotide + H2O
-
137054
Rattus norvegicus
?
-
-
-
?
3.2.2.5
NAD+ + H2O
-
137054
Rattus norvegicus
ADPribose + nicotinamide
-
-
-
?
3.2.2.5
NADP+ + H2O
-
137054
Rattus norvegicus
phospho-ADPribose + nicotinamide
-
-
-
?
3.2.2.5
NMN + H2O
-
137054
Rattus norvegicus
phosphoribose + nicotinamide
-
-
-
?
3.2.2.5
thioNAD+ + H2O
-
137054
Rattus norvegicus
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.5
6.5
7
chromatin
Rattus norvegicus
3.2.2.5
6.5
-
nuclear envelope
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.2.5
4.5
-
5 min, 60°C, retains of 55% of activity
Rattus norvegicus