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Literature summary extracted from
- ADP-ribosylarginine hydrolases and ADP-ribosyltransferases (1997), Adv. Exp. Med. Biol., 419, 25-33.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.19 | dithiothreitol | enhances, activation is maximal with 5-10 mM dithiothreitol and 5-10 mM MgCl2 | Meleagris gallopavo |  |
| 3.2.2.19 | dithiothreitol | synergistically stimulated by Mg2+ and dithiothreitol | Mus musculus | |
| 3.2.2.19 | dithiothreitol | synergistically stimulated by Mg2+ and dithiothreitol | Rattus norvegicus | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.2.19 | expression as a glutathione S-transferase-linked fusion protein in Escherichia coli | Rattus norvegicus |
| 3.2.2.19 | expression in Escherichia coli | Mus musculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.19 | ADP | inhibition by ADP stronger than by AMP | Meleagris gallopavo | |
| 3.2.2.19 | ADP | - |
Rattus norvegicus | |
| 3.2.2.19 | ADPribose | inhibition by ADPribose stronger than by ADP and AMP | Meleagris gallopavo | |
| 3.2.2.19 | ADPribose | - |
Oryctolagus cuniculus | |
| 3.2.2.19 | ADPribose | - |
Rattus norvegicus | |
| 3.2.2.19 | AMP | - |
Meleagris gallopavo | |
| 3.2.2.19 | NaCl | inhibition with 200 mM | Meleagris gallopavo | |
| 3.2.2.19 | NaF | more than 80% inhibition with 5 mM | Meleagris gallopavo | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.19 | Mg2+ | - |
Meleagris gallopavo | |
| 3.2.2.19 | Mg2+ | - |
Cavia porcellus | |
| 3.2.2.19 | Mg2+ | - |
Mus musculus | |
| 3.2.2.19 | Mg2+ | - |
Rattus norvegicus | |
| 3.2.2.19 | Mg2+ | - |
Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.19 | 39000 | - |
gel filtration, SDS-PAGE | Meleagris gallopavo |
| 3.2.2.19 | 39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Meleagris gallopavo |
| 3.2.2.19 | 39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Mus musculus |
| 3.2.2.19 | 39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Rattus norvegicus |
| 3.2.2.19 | 39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.19 | ADP-ribose-arginine + H2O | Meleagris gallopavo | - |
ADP-ribose + arginine | - |
? | |
| 3.2.2.19 | ADP-ribose-arginine + H2O | Rhodospirillum rubrum | the existence of a ADP-ribosylation cycle was established in the bacterium Rhodospirillium rubrum where it regulates dinitrogenase reductase, a key enzyme in nitrogen fixation | ADP-ribose + arginine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.2.19 | Bos taurus | - |
- |
- |
| 3.2.2.19 | Cavia porcellus | - |
- |
- |
| 3.2.2.19 | Meleagris gallopavo | - |
- |
- |
| 3.2.2.19 | Mus musculus | - |
- |
- |
| 3.2.2.19 | Oryctolagus cuniculus | - |
- |
- |
| 3.2.2.19 | Ovis aries | - |
- |
- |
| 3.2.2.19 | Rattus norvegicus | - |
- |
- |
| 3.2.2.19 | Rhodospirillum rubrum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.2.19 | 20000 fold by DE-52, phenyl-Sepharose, hydroxylapatite, Affi-Gel 501 organo-mercurial agarose, Ultrogel AcA 54 | Rattus norvegicus |
| 3.2.2.19 | 200000fold by DE-52, phenyl-Sepharose, hydroxylapatite, Ultrogel AcA 54, Mono Q chromatography | Meleagris gallopavo |
| 3.2.2.19 | partial | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.2.19 | brain | - |
Cavia porcellus | - |
| 3.2.2.19 | brain | - |
Mus musculus | - |
| 3.2.2.19 | brain | - |
Rattus norvegicus | - |
| 3.2.2.19 | brain | - |
Bos taurus | - |
| 3.2.2.19 | brain | - |
Oryctolagus cuniculus | - |
| 3.2.2.19 | brain | - |
Ovis aries | - |
| 3.2.2.19 | erythrocyte | - |
Meleagris gallopavo | - |
| 3.2.2.19 | spleen | - |
Rattus norvegicus | - |
| 3.2.2.19 | testis | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.19 | ADP-ribose-arginine + H2O | - |
Meleagris gallopavo | ADP-ribose + arginine | - |
? | |
| 3.2.2.19 | ADP-ribose-arginine + H2O | the existence of a ADP-ribosylation cycle was established in the bacterium Rhodospirillium rubrum where it regulates dinitrogenase reductase, a key enzyme in nitrogen fixation | Rhodospirillum rubrum | ADP-ribose + arginine | - |
? | |
| 3.2.2.19 | alpha-subunit of stimulating GTP-binding protein ADP-ribosylated + H2O | - |
Rattus norvegicus | alpha-subunit of stimulating GTP-binding protein + ADP-ribose | - |
? | |
| 3.2.2.19 | cholera toxin A1-subunit ADP-ribosylated + H2O | - |
Rattus norvegicus | cholera toxin A1-subunit + ADP-ribose | - |
? | |
| 3.2.2.19 | nonmuscle actin ADP-ribosylated + H2O | - |
Rattus norvegicus | nonmuscle actin + ADP-ribose | - |
? | |
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