EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.19 | dithiothreitol | - |
Mus musculus | |
3.2.2.19 | dithiothreitol | - |
Rattus norvegicus | |
3.2.2.19 | dithiothreitol | activated by Mg2+ and dithiothreitol | Meleagris gallopavo |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.19 | expression as a glutathione S-transferase-linked fusion protein in Escherichia coli | Rattus norvegicus |
3.2.2.19 | expression in Escherichia coli | Mus musculus |
3.2.2.19 | wild-type and mutant rat hydrolases are expressed in Escherichia coli | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.19 | ADP | inhibition by ADP stronger than by AMP | Meleagris gallopavo | |
3.2.2.19 | ADP | - |
Rattus norvegicus | |
3.2.2.19 | ADPribose | inhibition by ADPribose stronger than by ADP and AMP | Meleagris gallopavo | |
3.2.2.19 | ADPribose | - |
Oryctolagus cuniculus | |
3.2.2.19 | ADPribose | - |
Rattus norvegicus | |
3.2.2.19 | AMP | - |
Meleagris gallopavo | |
3.2.2.19 | dithiothreitol | incubation in 20 mM at 37°C leads to rapid loss of activity but addition of Mg2+ stabilizes the hydrolase against thermal inactivation | Meleagris gallopavo |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.19 | Mg2+ | - |
Meleagris gallopavo | |
3.2.2.19 | Mg2+ | - |
Mus musculus | |
3.2.2.19 | Mg2+ | - |
Rattus norvegicus | |
3.2.2.19 | Mg2+ | - |
Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.2.19 | 39000 | - |
- |
Mus musculus |
3.2.2.19 | 39000 | - |
- |
Rattus norvegicus |
3.2.2.19 | 39000 | - |
gel filtration, SDS-PAGE | Meleagris gallopavo |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.19 | ADP-ribose-arginine + H2O | Meleagris gallopavo | - |
ADP-ribose + arginine | - |
? | |
3.2.2.19 | ADP-ribose-arginine + H2O | Rhodospirillum rubrum | the existence of a ADP-ribosylation cycle was established in the bacterium Rhodospirillium rubrum where it regulates dinitrogenase reductase, a key enzyme in nitrogen fixation | ADP-ribose + arginine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.19 | Meleagris gallopavo | - |
- |
- |
3.2.2.19 | Mus musculus | - |
- |
- |
3.2.2.19 | Oryctolagus cuniculus | - |
- |
- |
3.2.2.19 | Ovis aries | - |
- |
- |
3.2.2.19 | Rattus norvegicus | - |
- |
- |
3.2.2.19 | Rhodospirillum rubrum | - |
- |
- |
3.2.2.19 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.19 | 20000 fold by DE-52, phenyl-Sepharose, hydroxylapatite, Affi-Gel 501 organo-mercurial agarose, Ultrogel AcA 54 | Rattus norvegicus |
3.2.2.19 | 200000fold by DE-52, phenyl-Sepharose, hydroxylapatite, Ultrogel AcA 54, Mono Q chromatography | Meleagris gallopavo |
3.2.2.19 | partial | Mus musculus |
3.2.2.19 | partial | Sus scrofa |
3.2.2.19 | partial | Oryctolagus cuniculus |
3.2.2.19 | partial | Ovis aries |
3.2.2.19 | partial by successive chromatography on DE-52 cellulose, phenyl-Sepharose and organomercurial agarose | Meleagris gallopavo |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.2.19 | erythrocyte | - |
Meleagris gallopavo | - |
3.2.2.19 | fibroblast | - |
Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.19 | ADP-ribose-arginine + H2O | - |
Meleagris gallopavo | ADP-ribose + arginine | - |
? | |
3.2.2.19 | ADP-ribose-arginine + H2O | the existence of a ADP-ribosylation cycle was established in the bacterium Rhodospirillium rubrum where it regulates dinitrogenase reductase, a key enzyme in nitrogen fixation | Rhodospirillum rubrum | ADP-ribose + arginine | - |
? | |
3.2.2.19 | histones ADP-ribosylated + H2O | - |
Mus musculus | ? | - |
? | |
3.2.2.19 | lysozyme ADP-ribosylated + H2O | - |
Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.19 | monomer | 1 * 39000, SDS-PAGE | Meleagris gallopavo |