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Literature summary extracted from

  • Sawai, T.; Ohara, S.; Ichimi, Y.; Okaji, S.; Hisada, K.; Fukaya, N.
    Purification and some properties of the isomaltodextranase of Actinomadura strain R10 and comparison with that of Arthrobacter globiformis T6 (1981), Carbohydr. Res., 89, 289-299.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.94 extracellular
-
 Arthrobacter globiformis
-
-
3.2.1.94 extracellular
-
 Actinomadura sp.
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.94 glucooligosaccharides + H2O Arthrobacter globiformis
-
 isomaltose + D-glucose
-
 ?
3.2.1.94 glucooligosaccharides + H2O Actinomadura sp.
-
 isomaltose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.94 Actinomadura sp.
-
-
-
3.2.1.94 Actinomadura sp. R10
-
-
-
3.2.1.94 Arthrobacter globiformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.94
-
 Actinomadura sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.94 culture filtrate
-
 Arthrobacter globiformis
-
3.2.1.94 culture filtrate
-
 Actinomadura sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.94 5
-
-
 Actinomadura sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.94 dextran + H2O
-
 Arthrobacter globiformis alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc
-
 ?
3.2.1.94 dextran + H2O
-
 Actinomadura sp. alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc
-
 ?
3.2.1.94 dextran + H2O
-
 Actinomadura sp. R10 alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc
-
 ?
3.2.1.94 glucooligosaccharides + H2O
-
 Arthrobacter globiformis isomaltose + D-glucose
-
 ?
3.2.1.94 glucooligosaccharides + H2O
-
 Actinomadura sp. isomaltose + D-glucose
-
 ?
3.2.1.94 isomaltotriitol + H2O
-
 Arthrobacter globiformis isomaltose + sorbitol
-
 ?
3.2.1.94 isomaltotriitol + H2O
-
 Actinomadura sp. isomaltose + sorbitol
-
 ?
3.2.1.94 isomaltotriitol + H2O
-
 Actinomadura sp. R10 isomaltose + sorbitol
-
 ?
3.2.1.94 isomaltotriose + H2O
-
 Arthrobacter globiformis alpha-isomaltose + D-glucose
-
 ?
3.2.1.94 isomaltotriose + H2O
-
 Actinomadura sp. alpha-isomaltose + D-glucose
-
 ?
3.2.1.94 isomaltotriose + H2O
-
 Actinomadura sp. R10 alpha-isomaltose + D-glucose
-
 ?
3.2.1.94 panose + H2O
-
 Arthrobacter globiformis alpha-isomaltose + D-glucose
-
 ?
3.2.1.94 panose + H2O
-
 Actinomadura sp. alpha-isomaltose + D-glucose
-
 ?
3.2.1.94 panose + H2O
-
 Actinomadura sp. R10 alpha-isomaltose + D-glucose
-
 ?
3.2.1.94 panose + H2O
-
 Arthrobacter globiformis isomaltose + D-glucose
-
 ?
3.2.1.94 panose + H2O
-
 Actinomadura sp. isomaltose + D-glucose
-
 ?
3.2.1.94 panose + H2O
-
 Actinomadura sp. R10 isomaltose + D-glucose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.94 4
-
-
 Arthrobacter globiformis
3.2.1.94 5
-
-
 Actinomadura sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.94 3 7.5 10% of maximum activity at pH 3 and 20% at pH 7.5 Actinomadura sp.