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Literature summary extracted from

  • Nakano, H.; Takenishi, S.; Watanabe, Y.
    Purification and properties of two galactanases from Penicillium citrinum (1985), Agric. Biol. Chem., 49, 3445-3454.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.89 galactanase I forms a hexagonal crystal Penicillium citrinum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.89 arabinose o-nitrophenyl-beta-D-galactoside hydrolyzing activity Penicillium citrinum
3.2.1.89 Fe3+
-
 Penicillium citrinum
3.2.1.89 galactose o-nitrophenyl-beta-D-galactoside hydrolyzing activity Penicillium citrinum
3.2.1.89 glucose o-nitrophenyl-beta-D-galactoside hydrolyzing activity Penicillium citrinum
3.2.1.89 Hg2+
-
 Penicillium citrinum
3.2.1.89 lactose o-nitrophenyl-beta-D-galactoside hydrolyzing activity Penicillium citrinum
3.2.1.89 xylose o-nitrophenyl-beta-D-galactoside hydrolyzing activity Penicillium citrinum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.89 extracellular
-
 Penicillium citrinum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.89 35000
-
-
 Penicillium citrinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.89 arabinogalactan + H2O Penicillium citrinum
-
 galactose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.89 Penicillium citrinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.89
-
 Penicillium citrinum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.89 224
-
 galactanase II Penicillium citrinum
3.2.1.89 238
-
 galactanase I Penicillium citrinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.89 arabinogalactan + H2O
-
 Penicillium citrinum galactose
-
 ?
3.2.1.89 beta-(1->4)-D-galactotriose + H2O by galactanase I Penicillium citrinum D-galactose
-
 ?
3.2.1.89 galactobiose + H2O by galactanase I Penicillium citrinum galactose
-
 ?
3.2.1.89 galactotetraose + H2O by galactanase I Penicillium citrinum galactose
-
 ?
3.2.1.89 p-nitrophenyl-beta-D-galactoside + H2O
-
 Penicillium citrinum p-nitrophenol + galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.89 monomer 1 * 35000 SDS-PAGE Penicillium citrinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.89 55
-
-
 Penicillium citrinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.89 55
-
 stable for 10 min at pH 5.5 Penicillium citrinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.89 4.5
-
-
 Penicillium citrinum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.89 4 10
-
 Penicillium citrinum