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Literature summary extracted from

  • Van Rijssel, M.; Gerwig, G.J.; Hansen, T.A.
    Isolation and characterisation of an extracellular glycosylated protein complex from Clostridium thermosacchalyticum with pectin methylesterase and polygalacturonate hydrolase activity (1993), Appl. Environ. Microbiol., 59, 828-836.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.82 0.02
-
 Pectin
-
 Thermoanaerobacterium thermosaccharolyticum
3.2.1.82 0.08
-
 pectate
-
 Thermoanaerobacterium thermosaccharolyticum
3.2.1.82 3.1
-
 tetragalacturonate
-
 Thermoanaerobacterium thermosaccharolyticum
3.2.1.82 3.1
-
 alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronic acid
-
 Thermoanaerobacterium thermosaccharolyticum
3.2.1.82 4.1
-
 hexagalacturonate
-
 Thermoanaerobacterium thermosaccharolyticum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.82 extracellular
-
 Thermoanaerobacterium thermosaccharolyticum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.82 pectin + H2O Thermoanaerobacterium thermosaccharolyticum
-
 digalacturonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.82 Thermoanaerobacterium thermosaccharolyticum
-
 protein complex with polygalacturonate hydrolase activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.82 partial Thermoanaerobacterium thermosaccharolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.82 alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronic acid + H2O
-
 Thermoanaerobacterium thermosaccharolyticum ?
-
 ?
3.2.1.82 hexagalacturonate + H2O
-
 Thermoanaerobacterium thermosaccharolyticum ?
-
 ?
3.2.1.82 pectate + H2O
-
 Thermoanaerobacterium thermosaccharolyticum digalacturonate
-
 ?
3.2.1.82 pectin + H2O
-
 Thermoanaerobacterium thermosaccharolyticum digalacturonate
-
 ?
3.2.1.82 polygalacturonate + H2O
-
 Thermoanaerobacterium thermosaccharolyticum digalacturonate
-
 ?
3.2.1.82 tetragalacturonate + H2O
-
 Thermoanaerobacterium thermosaccharolyticum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.82 70
-
 pectate Thermoanaerobacterium thermosaccharolyticum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.82 40 70 activity decreases rapidly at higher temperatures Thermoanaerobacterium thermosaccharolyticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.82 70
-
 stable for more than 1 h Thermoanaerobacterium thermosaccharolyticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.82 5
-
 pectate Thermoanaerobacterium thermosaccharolyticum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.82 2.5 7 pectate Thermoanaerobacterium thermosaccharolyticum