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Literature summary extracted from

  • Pitson, S.M.; Seviour, R.J.; McDougall, B.M.; Stone, B.A.; Sadek, M.
    Purification and characterization of an extracellular (1,6)-beta-glucanase from the filamentous fungus Acremonium persicinum (1996), Biochem. J., 316, 841-846.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.75 2-Hydroxy-5-nitrobenzyl bromide
-
 Acremonium persicinum
3.2.1.75 Cu2+
-
 Acremonium persicinum
3.2.1.75 Dicyclohexylcarbodiimide
-
 Acremonium persicinum
3.2.1.75 Hg2+
-
 Acremonium persicinum
3.2.1.75 KMnO4
-
 Acremonium persicinum
3.2.1.75 MgSO4
-
 Acremonium persicinum
3.2.1.75 Mn2+
-
 Acremonium persicinum
3.2.1.75 N-Acetylimidazole
-
 Acremonium persicinum
3.2.1.75 N-bromosuccinimide
-
 Acremonium persicinum
3.2.1.75 N-ethyl-5-phenylisoxazolium 3'-sulfonate
-
 Acremonium persicinum
3.2.1.75 p-hydroxymercuribenzoate
-
 Acremonium persicinum
3.2.1.75 Sn2+
-
 Acremonium persicinum
3.2.1.75 Zn2+
-
 Acremonium persicinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.75 additional information
-
 additional information lutean 1.38 mg/ml, laminarin 1.67 mg/ml Acremonium persicinum
3.2.1.75 0.0753
-
 pustulan assumed molecular weight for pustulan 17000 Da, or KM of 1.28 mg/ml Acremonium persicinum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.75 extracellular
-
 Acremonium persicinum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.75 42700
-
 x * 42700, SDS-PAGE Acremonium persicinum
3.2.1.75 44300
-
 gel filtration Acremonium persicinum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.75 Acremonium persicinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.75 89fold Acremonium persicinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.75 culture filtrate
-
 Acremonium persicinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.75 146
-
-
 Acremonium persicinum

Storage Stability

EC Number Storage Stability Organism
3.2.1.75 4°C, 4 months, no loss of activity, -70°C, 12 months, no loss of activity Acremonium persicinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.75 beta-glucan + H2O yeast glucans Acremonium persicinum glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
 ?
3.2.1.75 laminarin + H2O
-
 Acremonium persicinum gentiobiose + D-glucose
-
 ?
3.2.1.75 lutean + H2O
-
 Acremonium persicinum D-glucose + gentiobiose + gentiotriose
-
 ?
3.2.1.75 pustulan + H2O
-
 Acremonium persicinum glucose + gentiobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.75 ? x * 42700, SDS-PAGE Acremonium persicinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.75 50
-
 short-term stability up to 50°C Acremonium persicinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.75 5
-
-
 Acremonium persicinum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.75 4.5 9.5
-
 Acremonium persicinum