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Literature summary extracted from

  • Rombouts, F.M.; Phaff, H.J.
    Lysis of yeast cell walls. Lytic beta-(1-6)-glucanase from Bacillus circulans WL-12 (1976), Eur. J. Biochem., 63, 109-120.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.75 additional information
-
 additional information pustulan 0.29 mg/ml Niallia circulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.75 extracellular
-
 Niallia circulans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.75 54000
-
 SDS-PAGE, gel filtration Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.75 Niallia circulans
-
 WL-12
-
3.2.1.75 Niallia circulans WL-12
-
 WL-12
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.75 culture filtrate
-
 Niallia circulans
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.75 -25°C, 1 month, less than 10% loss of activity Niallia circulans
3.2.1.75 2°C, 1 week, 20% loss of activity Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.75 beta-glucan + H2O yeast glucans Niallia circulans glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
 ?
3.2.1.75 beta-glucan + H2O yeast glucans Niallia circulans WL-12 glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
 ?
3.2.1.75 gentiotetraose + H2O
-
 Niallia circulans D-glucose + gentiotriose + gentiobiose
-
 ?
3.2.1.75 gentiotetraose + H2O
-
 Niallia circulans WL-12 D-glucose + gentiotriose + gentiobiose
-
 ?
3.2.1.75 laminarin + H2O
-
 Niallia circulans gentiobiose + D-glucose
-
 ?
3.2.1.75 p-nitrophenyl-beta-D-glucoside + H2O
-
 Niallia circulans 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.75 p-nitrophenyl-beta-D-glucoside + H2O
-
 Niallia circulans WL-12 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.75 pachyman + H2O poor substrate Niallia circulans gentiobiose trace ?
3.2.1.75 pachyman + H2O poor substrate Niallia circulans WL-12 gentiobiose trace ?
3.2.1.75 pustulan + H2O
-
 Niallia circulans glucose + gentiobiose also gentiopentaose, gentiotetraose and gentiotriose ?
3.2.1.75 pustulan + H2O
-
 Niallia circulans WL-12 glucose + gentiobiose also gentiopentaose, gentiotetraose and gentiotriose ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.75 6
-
 in Tris/succinate buffer Niallia circulans
3.2.1.75 6.5
-
 in sodium phosphate buffer Niallia circulans