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Literature summary extracted from
- Cellobiosidase from Ruminococcus albus (1988), Methods Enzymol., 160, 391-398.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.74 | Cu2+ | - |
Ruminococcus albus |  |
| 3.2.1.74 | iodoacetoamide | - |
Ruminococcus albus | |
| 3.2.1.74 | p-chloromercuribenzoate | - |
Ruminococcus albus | |
| 3.2.1.74 | Zn2+ | - |
Ruminococcus albus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.74 | extracellular | - |
Ruminococcus albus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 100000 | - |
alpha,beta, 2 * 100000, SDS-PAGE | Ruminococcus albus |
| 3.2.1.74 | 200000 | - |
gel filtration | Ruminococcus albus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.74 | Ruminococcus albus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.74 | 39fold | Ruminococcus albus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.74 | culture medium | - |
Ruminococcus albus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | additional information | - |
20.8 OD405/mg | Ruminococcus albus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.74 | Avicel + H2O | - |
Ruminococcus albus | D-glucose + cellotetraose + cellotriose + cellobiose | - |
? | |
| 3.2.1.74 | carboxymethyl cellulose + H2O | - |
Ruminococcus albus | ? | - |
? | |
| 3.2.1.74 | cellobiose + H2O | - |
Ruminococcus albus | D-glucose + cellotetraose + cellotriose | - |
ir | |
| 3.2.1.74 | cellooligomers + H2O | - |
Ruminococcus albus | cellobiose + cellooligomers | - |
ir | |
| 3.2.1.74 | cellopentaose + H2O | - |
Ruminococcus albus | D-glucose + ? | - |
ir | |
| 3.2.1.74 | cellotetraose + H2O | - |
Ruminococcus albus | D-glucose + cellotriose + cellobiose | - |
ir | |
| 3.2.1.74 | cellotriose + H2O | - |
Ruminococcus albus | D-glucose + cellobiose | - |
ir | |
| 3.2.1.74 | cellulose + H2O | ball-milled | Ruminococcus albus | D-glucose + ? | - |
? | |
| 3.2.1.74 | p-nitrophenyl-beta-D-cellobioside + H2O | - |
Ruminococcus albus | cellobiose + p-nitrophenol | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.74 | dimer | alpha,beta, 2 * 100000, SDS-PAGE | Ruminococcus albus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 37 | - |
- |
Ruminococcus albus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 30 | - |
stable below 30°C | Ruminococcus albus |
| 3.2.1.74 | 37 | - |
20% activity loss after 10 min, 75% activity loss after 15 h at 37°C | Ruminococcus albus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 6.8 | - |
- |
Ruminococcus albus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 5.5 | 8 | - |
Ruminococcus albus |
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