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Literature summary extracted from

  • Zhang, S.; Irwin, D.C.; Wilson, D.B.
    Site-directed mutation of noncatalytic residues of Thermobifida fusca exocellulase Cel6B (2000), Eur. J. Biochem., 267, 3101-3115.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.74 Escherichia coli and Streptomyces lividans Thermobifida fusca

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.74 A229V loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 E495D active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 E495N active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 G234A loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 G234D loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 G234N loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 G234S loop mutant, higher binding affinities for 4-methylumbelliferyl beta-cellotrioside and cellotriose Thermobifida fusca
3.2.1.74 G234S/G284P loop mutant, higher binding affinity for 4-methylumbelliferyl beta-cellotrioside Thermobifida fusca
3.2.1.74 G284A loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 G284I loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 G284P loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 G284P/A229V loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 G331A active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 G496P loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 H326A active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 H326S active site mutant, higher binding affinities for 4-methylumbelliferyl beta-cellobioside and cellotriose Thermobifida fusca
3.2.1.74 L230C loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 L230C/D512C loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 N233C loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 N233C/D506C loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 W329C active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 W332A active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
3.2.1.74 W332Y active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.74 D-cellobiose competitive inhibition Thermobifida fusca

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.74 extracellular
-
Thermobifida fusca
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.74 Thermobifida fusca
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.74 culture medium
-
Thermobifida fusca
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.74 4-methylumbelliferyl 4-O-(beta-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
-
Thermobifida fusca ?
-
?
3.2.1.74 4-methylumbelliferyl beta-cellobioside + H2O
-
Thermobifida fusca 4-methylumbelliferone + cellobiose
-
?
3.2.1.74 4-methylumbelliferyl beta-cellotrioside + H2O
-
Thermobifida fusca 4-methylumbelliferone + cellotriose
-
?
3.2.1.74 4-methylumbelliferyl beta-D-glucopyranoside + H2O
-
Thermobifida fusca 4-methylumbelliferone + D-glucopyranose
-
?
3.2.1.74 carboxymethyl cellulose + H2O
-
Thermobifida fusca ?
-
?
3.2.1.74 cellopentaose + H2O
-
Thermobifida fusca D-glucose + ?
-
?
3.2.1.74 cellotetraose + H2O
-
Thermobifida fusca D-glucose + cellotriose + cellobiose
-
?
3.2.1.74 cellotriose + H2O
-
Thermobifida fusca D-glucose + cellobiose
-
?
3.2.1.74 cellulose + H2O H3PO4 swollen Thermobifida fusca D-glucose + ?
-
?
3.2.1.74 cellulose + H2O bacterial microcrystalline cellulose Thermobifida fusca D-glucose + ?
-
?