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Literature summary extracted from

  • Nakamura, T.; Shitara, A.; Matsulda, S.; Matsuo, T.; Suiko, M.; Ohta, K.
    Production, purification and properties of an endoinulinase of Penicillium sp. TN-88 that liberates inulotriose (1997), J. Ferment. Bioeng., 84, 313-318.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.7 Ag+
-
 Penicillium sp.
3.2.1.7 carbodiimide
-
 Penicillium sp.
3.2.1.7 EDTA
-
 Penicillium sp.
3.2.1.7 Fe3+
-
 Penicillium sp.
3.2.1.7 Hg2+
-
 Penicillium sp.
3.2.1.7 N-bromosuccinimide
-
 Penicillium sp.
3.2.1.7 PCMB
-
 Penicillium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.7 0.2
-
 inulin at 40°C, pH 5.0 Penicillium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.7 Mn2+ stimulates Penicillium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Penicillium sp.
-
 TN-88
-
3.2.1.7 Penicillium sp. TN-88
-
 TN-88
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.7 glycoprotein contains 1.4% carbohydrate Penicillium sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.7
-
 Penicillium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.7 105
-
-
 Penicillium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O
-
 Penicillium sp. oligofructosides inulotriose and inulotetraose are liberated as the main products after 24 h, prolonged reaction yields inulotriose as the main product ?
3.2.1.7 inulin + H2O
-
 Penicillium sp. TN-88 oligofructosides inulotriose and inulotetraose are liberated as the main products after 24 h, prolonged reaction yields inulotriose as the main product ?
3.2.1.7 additional information no activity with levan Penicillium sp. ?
-
 ?
3.2.1.7 additional information no activity with sucrose Penicillium sp. ?
-
 ?
3.2.1.7 additional information no activity with raffinose Penicillium sp. ?
-
 ?
3.2.1.7 additional information no activity with levan Penicillium sp. TN-88 ?
-
 ?
3.2.1.7 additional information no activity with sucrose Penicillium sp. TN-88 ?
-
 ?
3.2.1.7 additional information no activity with raffinose Penicillium sp. TN-88 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.7 40
-
 30 min, stable Penicillium sp.
3.2.1.7 60
-
 30 min, complete inactivation Penicillium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 5.2
-
-
 Penicillium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.7 5 7 24 h, stable Penicillium sp.