Any feedback?
Literature summary extracted from
- Molecular weight of the undegraded polypeptide chain of Pseudomonas amyloderamosa isoamylase (1980), Biochim. Biophys. Acta, 611, 390-393.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.68 | - |
Pseudomonas amyloderamosa |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 46000 | - |
2 * 46000, sedimentation equilibrium | Pseudomonas amyloderamosa |
| 3.2.1.68 | 52000 | - |
2 * 52000, gel filtration | Pseudomonas amyloderamosa |
| 3.2.1.68 | 86000 | 94000 | 86000, undegraded polypeptide chain, SDS-PAGE | Pseudomonas amyloderamosa |
| 3.2.1.68 | 88000 | - |
calculated by comparison with standard proteins | Pseudomonas amyloderamosa |
| 3.2.1.68 | 90000 | - |
undegraded polypeptide chain | Pseudomonas amyloderamosa |
| 3.2.1.68 | 94000 | - |
native enzyme, centrifugation analysis | Pseudomonas amyloderamosa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.68 | starch + H2O | Pseudomonas amyloderamosa | - |
? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.68 | Pseudomonas amyloderamosa | - |
strain KlC, revertant strain of Kl | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.68 | amylopectin + H2O | or the beta-limit dextrins, cleaves the branching points completely in vitro | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
| 3.2.1.68 | glycogen + H2O | cleaves the branching points completely in vitro | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
| 3.2.1.68 | glycogen + H2O | or the beta-limit dextrins | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
| 3.2.1.68 | starch + H2O | - |
Pseudomonas amyloderamosa | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.68 | dimer | 2 * 46000, sedimentation equilibrium | Pseudomonas amyloderamosa |
| 3.2.1.68 | dimer | 2 * 52000, gel filtration | Pseudomonas amyloderamosa |
| 3.2.1.68 | dimer | dimers probably obtained by partial proteolytic degradation | Pseudomonas amyloderamosa |
| 3.2.1.68 | monomer | gel electrophoresis | Pseudomonas amyloderamosa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
