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Literature summary extracted from

  • Amemura, A.; Chakraborty, R.; Fujita, M.; Noumi, T.; Masamitsu, F.
    Cloning and nucleotide sequence of the isoamylase gene from Pseudomonas amyloderamosa SB-15 (1988), J. Biol. Chem., 263, 9271-9275.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.68 analysis elucidating of the finestructures of various polysaccharides Pseudomonas amyloderamosa
3.2.1.68 nutrition industrial production of amylose, maltose and D-glucose from starch, alone or in combination with beta-amylase and glucoamylase production Pseudomonas amyloderamosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.68
-
 Pseudomonas amyloderamosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.68
-
 Pseudomonas amyloderamosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.68 extracellular
-
 Pseudomonas amyloderamosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.68 80810
-
 SB-15, DNA sequence analysis Pseudomonas amyloderamosa
3.2.1.68 86000 94000 SB-15, gel filtration, SDS-PAGE Pseudomonas amyloderamosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.68 starch + H2O Pseudomonas amyloderamosa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.68 Pseudomonas amyloderamosa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.68 alpha-D-glucan + H2O cleavage of alpha-D-glucosidic linkages of certain branched alpha-D-glucans Pseudomonas amyloderamosa ?
-
 ?
3.2.1.68 amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
 ?
3.2.1.68 glycogen + H2O cleaves the branching points completely in vitro Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
 ?
3.2.1.68 glycogen + H2O or the beta-limit dextrins Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
 ?
3.2.1.68 additional information direct debranching enzyme, attacks unmodified glycogen and/or amylopectin with hydrolysis of the 1,6-bond, smallest substrate is not known Pseudomonas amyloderamosa ?
-
 ?
3.2.1.68 additional information distinguished from alpha-dextrin endo-1,6-alpha-glucosidase, EC 3.2.1.41, by the inability of isoamylase to attack pullulan, and by limited action on alpha-limit dextrins, action on glycogen however is complete in contrast to limited action by alpha-dextrin glucanohydrolase, 1,6-linkage hydrolysed only at branch point Pseudomonas amyloderamosa ?
-
 ?
3.2.1.68 starch + H2O
-
 Pseudomonas amyloderamosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.68 glycogen 6-glucanohydrolase
-
 Pseudomonas amyloderamosa