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Literature summary extracted from

  • Kester, H.C.M.; Kusters-Van Someren, M.A.; Mueller, Y.; Visser, J.
    Primary structure and characterization of an exopolygalacturonase from Aspergillus tubingensis (1996), Eur. J. Biochem., 240, 738-746.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.67 synthesis pectic enzymes industrially important in processing of agricultural products, production of pectinolytic enzymes, specifical enzyme for modification of pectins Aspergillus tubingensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.67 gene pgaX cloned by reverse genetics, pIM361, overexpression of the enzyme by transformants Aspergillus tubingensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 D-galactopyranuronic acid catabolite repression, completely inhibited, inhibitory constant 0.3 mM Aspergillus tubingensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 0.32
-
 (1,4-alpha-D-galacturonide)7
-
 Aspergillus tubingensis
3.2.1.67 0.44
-
 (1,4-alpha-D-galacturonide)6
-
 Aspergillus tubingensis
3.2.1.67 0.53
-
 (1,4-alpha-D-galacturonide)5
-
 Aspergillus tubingensis
3.2.1.67 0.68
-
 (1,4-alpha-D-galacturonide)3
-
 Aspergillus tubingensis
3.2.1.67 0.94
-
 (1,4-alpha-D-galacturonide)3
-
 Aspergillus tubingensis
3.2.1.67 1.44
-
 (1,4-alpha-D-galacturonide)2
-
 Aspergillus tubingensis
3.2.1.67 3.1
-
 (1,4-alpha-D-galacturonide)n recombinant enzyme Aspergillus tubingensis
3.2.1.67 3.2
-
 (1,4-alpha-D-galacturonide)n wild type enzyme Aspergillus tubingensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 extracellular
-
 Aspergillus tubingensis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 78000
-
 SDS-PAGE Aspergillus tubingensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Aspergillus tubingensis
-
 filamentous fungus, wild-type strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 wild-type and recombinant enzyme Aspergillus tubingensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 (1,4-alpha-D-galacturonide)2 + H2O
-
 Aspergillus tubingensis D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)3 + H2O
-
 Aspergillus tubingensis galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)4 + H2O
-
 Aspergillus tubingensis D-galacturonate
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)5 + H2O
-
 Aspergillus tubingensis D-galacturonic acid + (1,4-alpha-D-galacturonide)2 + (1,4-alpha-D-galacturonide)3 + (1,4-alpha-D-galacturonide)4
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)6 + H2O
-
 Aspergillus tubingensis D-galacturonic acid + (1,4-alpha-D-galacturonide)5
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)7 + H2O
-
 Aspergillus tubingensis ?
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)n + H2O
-
 Aspergillus tubingensis D-galacturonate + digalacturonic acid + trigalacturonic acid
-
 ?
3.2.1.67 oligo-D-galacturonate + H2O
-
 Aspergillus tubingensis D-galacturonic acid
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.67 30 50 50°C 50% activity retained Aspergillus tubingensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 4.2
-
-
 Aspergillus tubingensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.67 3 5
-
 Aspergillus tubingensis