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Literature summary extracted from

  • Kang, E.J.; Lee, S.O.; Lee, J.D.; Lee, T.H.
    Purification and characterization of a levanbiose-producing levanase from Pseudomonas sp. No. 43 (1999), Biotechnol. Appl. Biochem., 29, 263-268.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.65 agriculture plant differentiation, growth promoting activities for some enteric bacteria Pseudomonas sp. No. 43
3.2.1.65 medicine enhancing effect on the immune system and other physiological functions Pseudomonas sp. No. 43
3.2.1.65 nutrition energy source Pseudomonas sp. No. 43
3.2.1.65 nutrition production of noncaloric sweeteners Pseudomonas sp. No. 43
3.2.1.65 synthesis protection from aging Pseudomonas sp. No. 43

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.65 2-mercaptoethanol
-
 Pseudomonas sp. No. 43
3.2.1.65 Ag+
-
 Pseudomonas sp. No. 43
3.2.1.65 Al3+
-
 Pseudomonas sp. No. 43
3.2.1.65 Ammonium oxalate
-
 Pseudomonas sp. No. 43
3.2.1.65 ascorbic acid
-
 Pseudomonas sp. No. 43
3.2.1.65 Ba2+
-
 Pseudomonas sp. No. 43
3.2.1.65 Ca2+
-
 Pseudomonas sp. No. 43
3.2.1.65 Co2+
-
 Pseudomonas sp. No. 43
3.2.1.65 EDTA
-
 Pseudomonas sp. No. 43
3.2.1.65 KMnO4
-
 Pseudomonas sp. No. 43
3.2.1.65 Mg2+
-
 Pseudomonas sp. No. 43
3.2.1.65 Mn2+
-
 Pseudomonas sp. No. 43
3.2.1.65 Sodium thioglycollate
-
 Pseudomonas sp. No. 43

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.65 extracellular
-
 Pseudomonas sp. No. 43
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.65 36000
-
 gel filtration Pseudomonas sp. No. 43

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.65 Pseudomonas sp. No. 43
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.65
-
 Pseudomonas sp. No. 43

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.65 30.5
-
 U/mg Pseudomonas sp. No. 43

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.65 3 levan + 7 H2O
-
 Pseudomonas sp. No. 43 10 levanbiose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.65 40
-
-
 Pseudomonas sp. No. 43

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.65 40
-
 stable up to 50°C but rapidly inactivated above Pseudomonas sp. No. 43

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.65 7
-
-
 Pseudomonas sp. No. 43

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.65 6 8 20°C, stable up to 50°C at pH 7.0 Pseudomonas sp. No. 43
3.2.1.65 6 8 activity decreases rapidly below pH 6.0 and above pH 8.0 Pseudomonas sp. No. 43