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Literature summary extracted from

  • Saito, K.; Kondo, K.; Kojima, I.; Yokota, A.; Tomita, F.
    Purification and characterization of 2,6-beta-D-fructan 6-levanbiohydrolase from Streptomyces exfoliatus F3-2 (2000), Appl. Environ. Microbiol., 66, 252-256.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.64 extracellular
-
 Streptomyces exfoliatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.64 54000
-
 1 * 54000, SDS-PAGE Streptomyces exfoliatus
3.2.1.64 60000
-
 gel filtration Streptomyces exfoliatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.64 3 levan + 7 H2O Streptomyces exfoliatus levan i.e. 2,6-beta-D-fructan 10 levanbiose 6-beta-fructosylfructose ?
3.2.1.64 3 levan + 7 H2O Streptomyces exfoliatus F3-2 levan i.e. 2,6-beta-D-fructan 10 levanbiose 6-beta-fructosylfructose ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.64 Streptomyces exfoliatus
-
-
-
3.2.1.64 Streptomyces exfoliatus F3-2
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.64 culture supernatant
-
 Streptomyces exfoliatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.64 105
-
-
 Streptomyces exfoliatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.64 1-fructosylnystose + H2O at 81.4% the rate of levan Streptomyces exfoliatus beta-D-fructose + alpha-D-glucose + sucrose + 1-kestose + nystose
-
 ?
3.2.1.64 1-kestose + H2O at 33.3% the rate of levan Streptomyces exfoliatus sucrose + beta-D-fructose + alpha-D-glucose
-
 ?
3.2.1.64 1-kestose + H2O at 33.3% the rate of levan Streptomyces exfoliatus F3-2 sucrose + beta-D-fructose + alpha-D-glucose
-
 ?
3.2.1.64 3 levan + 7 H2O levan i.e. 2,6-beta-D-fructan Streptomyces exfoliatus 10 levanbiose 6-beta-fructosylfructose ?
3.2.1.64 3 levan + 7 H2O smallest substrate to be hydrolyzed is levantriose Streptomyces exfoliatus 10 levanbiose 6-beta-fructosylfructose ?
3.2.1.64 3 levan + 7 H2O levan i.e. 2,6-beta-D-fructan Streptomyces exfoliatus F3-2 10 levanbiose 6-beta-fructosylfructose ?
3.2.1.64 3 levan + 7 H2O smallest substrate to be hydrolyzed is levantriose Streptomyces exfoliatus F3-2 10 levanbiose 6-beta-fructosylfructose ?
3.2.1.64 nystose + H2O at 73.4% the rate of levan Streptomyces exfoliatus beta-D-fructose + alpha-D-glucose + sucrose + 1-kestose
-
 ?
3.2.1.64 nystose + H2O at 73.4% the rate of levan Streptomyces exfoliatus F3-2 beta-D-fructose + alpha-D-glucose + sucrose + 1-kestose
-
 ?
3.2.1.64 sucrose + H2O at 6.5% the rate of levan Streptomyces exfoliatus beta-D-fructose + alpha-D-glucose
-
 ?
3.2.1.64 sucrose + H2O at 6.5% the rate of levan Streptomyces exfoliatus F3-2 beta-D-fructose + alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.64 monomer 1 * 54000, SDS-PAGE Streptomyces exfoliatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.64 50
-
 stable up to Streptomyces exfoliatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.64 5.5
-
-
 Streptomyces exfoliatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.64 3.5 8
-
 Streptomyces exfoliatus