BRENDA - Enzyme Database

Purification and properties of alpha-L-fucosidase from Bacillus fulminans

Kochibe, N.; J. Biochem. 74, 1141-1149 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
deoxycholate
34% inhibition at 0.2%, w/v
Bacillus fulminans
3.2.1.51
ethylenediaminetetraacetic acid
68% inhibition at 0.1 mM
Bacillus fulminans
3.2.1.51
Hg2+
marked inhibition at 0.1 mM
Bacillus fulminans
3.2.1.51
Mn2+
slight inhibition at 0.1 mM
Bacillus fulminans
3.2.1.51
SDS
42% inhibition at 0.02%, w/v
Bacillus fulminans
3.2.1.63
deoxycholate
-
Bacillus fulminans
3.2.1.63
EDTA
-
Bacillus fulminans
3.2.1.63
Hg2+
-
Bacillus fulminans
3.2.1.63
Mn2+
-
Bacillus fulminans
3.2.1.63
SDS
-
Bacillus fulminans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.33
-
porcine submaxillary mucine, desialyzed
-
Bacillus fulminans
3.2.1.51
1.6
-
glycopeptide from desialyzed porcine submaxillary mucin
-
Bacillus fulminans
3.2.1.51
2.5
-
2'-fucosyllactose
-
Bacillus fulminans
3.2.1.63
0.175
-
glycoprotein
hog, submaxillary
Bacillus fulminans
3.2.1.63
0.33
-
porcine submaxillary mucin
-
Bacillus fulminans
3.2.1.63
1.6
-
glycopeptide desialyzed porcine submaxillary mucin
-
Bacillus fulminans
3.2.1.63
2
-
alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4-)-beta-Glc
-
Bacillus fulminans
3.2.1.63
2.5
-
2'-fucosyllactose
-
Bacillus fulminans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
intracellular
-
Bacillus fulminans
5622
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
70000
80000
gel filtration
Bacillus fulminans
3.2.1.63
70000
80000
gel filtration
Bacillus fulminans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Bacillus fulminans
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Bacillus fulminans
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Bacillus fulminans
2'-fucosyllactose
alpha-L-fucose + lactose
-
Bacillus fulminans
?
3.2.1.51
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O
Bacillus fulminans
-
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
-
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
Bacillus fulminans
desialyzed porcine submaxillary mucin
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
Bacillus fulminans
meconium H-1, H-2 and X-1
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
glycoprotein + H2O
Bacillus fulminans
specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
meconium H-1 + H2O
Bacillus fulminans
-
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
meconium H-2 + H2O
Bacillus fulminans
-
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
meconium X-1 + H2O
Bacillus fulminans
low activity
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
submaxillary mucin + H2O
Bacillus fulminans
desialyzed and not, desialyzed mucine best substrate tested
alpha-L-fucose + ?
-
Bacillus fulminans
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Bacillus fulminans
-
-
-
3.2.1.63
Bacillus fulminans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
near homogeneity, chromatography techniques
Bacillus fulminans
3.2.1.63
-
Bacillus fulminans
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Bacillus fulminans
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
0.12
-
-
Bacillus fulminans
3.2.1.63
0.12
-
-
Bacillus fulminans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
136753
Bacillus fulminans
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
136753
Bacillus fulminans
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
136753
Bacillus fulminans
alpha-L-fucose + lactose
-
136753
Bacillus fulminans
?
3.2.1.51
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O
-
136753
Bacillus fulminans
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
-
136753
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
from meconium H-1, H-2 and X-1
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
desialyzed porcine submaxillry mucin
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
desialyzed porcine submaxillary mucin
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
meconium H-1, H-2 and X-1
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
glycoprotein + H2O
specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
meconium H-1 + H2O
-
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
meconium H-2 + H2O
-
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
meconium X-1 + H2O
low activity
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
stomach + H2O
-
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
submaxillary mucin + H2O
desialyzed and not, desialyzed mucine best substrate tested
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.63
2'-fucosyllactose + H2O
-
136753
Bacillus fulminans
fucose + lactose
-
-
-
-
3.2.1.63
lacto-N-fucopentaose I
-
136753
Bacillus fulminans
?
-
-
-
-
3.2.1.63
mucin + H2O
porcine submaxillary and glycopeptide of desialyzed mucin
136753
Bacillus fulminans
L-fucose + ?
-
-
-
-
3.2.1.63
O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O
i.e. 2-O-alpha-L-fucosyllactose
136753
Bacillus fulminans
L-fucose + lactose
-
136753
Bacillus fulminans
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
7% loss of activity after 5 min
Bacillus fulminans
3.2.1.51
70
-
77% loss of activity after 5 min
Bacillus fulminans
3.2.1.63
50
-
5 min, no loss of activity
Bacillus fulminans
3.2.1.63
60
-
5 min, 7% loss of activity
Bacillus fulminans
3.2.1.63
70
-
5 min, 77% loss of activity
Bacillus fulminans
3.2.1.63
80
-
5 min, complete loss of activity
Bacillus fulminans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
deoxycholate
34% inhibition at 0.2%, w/v
Bacillus fulminans
3.2.1.51
ethylenediaminetetraacetic acid
68% inhibition at 0.1 mM
Bacillus fulminans
3.2.1.51
Hg2+
marked inhibition at 0.1 mM
Bacillus fulminans
3.2.1.51
Mn2+
slight inhibition at 0.1 mM
Bacillus fulminans
3.2.1.51
SDS
42% inhibition at 0.02%, w/v
Bacillus fulminans
3.2.1.63
deoxycholate
-
Bacillus fulminans
3.2.1.63
EDTA
-
Bacillus fulminans
3.2.1.63
Hg2+
-
Bacillus fulminans
3.2.1.63
Mn2+
-
Bacillus fulminans
3.2.1.63
SDS
-
Bacillus fulminans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.33
-
porcine submaxillary mucine, desialyzed
-
Bacillus fulminans
3.2.1.51
1.6
-
glycopeptide from desialyzed porcine submaxillary mucin
-
Bacillus fulminans
3.2.1.51
2.5
-
2'-fucosyllactose
-
Bacillus fulminans
3.2.1.63
0.175
-
glycoprotein
hog, submaxillary
Bacillus fulminans
3.2.1.63
0.33
-
porcine submaxillary mucin
-
Bacillus fulminans
3.2.1.63
1.6
-
glycopeptide desialyzed porcine submaxillary mucin
-
Bacillus fulminans
3.2.1.63
2
-
alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4-)-beta-Glc
-
Bacillus fulminans
3.2.1.63
2.5
-
2'-fucosyllactose
-
Bacillus fulminans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
intracellular
-
Bacillus fulminans
5622
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
70000
80000
gel filtration
Bacillus fulminans
3.2.1.63
70000
80000
gel filtration
Bacillus fulminans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Bacillus fulminans
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Bacillus fulminans
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Bacillus fulminans
2'-fucosyllactose
alpha-L-fucose + lactose
-
Bacillus fulminans
?
3.2.1.51
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O
Bacillus fulminans
-
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
-
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
Bacillus fulminans
desialyzed porcine submaxillary mucin
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
Bacillus fulminans
meconium H-1, H-2 and X-1
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
glycoprotein + H2O
Bacillus fulminans
specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
meconium H-1 + H2O
Bacillus fulminans
-
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
meconium H-2 + H2O
Bacillus fulminans
-
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
meconium X-1 + H2O
Bacillus fulminans
low activity
alpha-L-fucose + ?
-
Bacillus fulminans
?
3.2.1.51
submaxillary mucin + H2O
Bacillus fulminans
desialyzed and not, desialyzed mucine best substrate tested
alpha-L-fucose + ?
-
Bacillus fulminans
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
near homogeneity, chromatography techniques
Bacillus fulminans
3.2.1.63
-
Bacillus fulminans
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Bacillus fulminans
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
0.12
-
-
Bacillus fulminans
3.2.1.63
0.12
-
-
Bacillus fulminans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
136753
Bacillus fulminans
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
136753
Bacillus fulminans
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
136753
Bacillus fulminans
alpha-L-fucose + lactose
-
136753
Bacillus fulminans
?
3.2.1.51
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O
-
136753
Bacillus fulminans
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
-
136753
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
from meconium H-1, H-2 and X-1
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
desialyzed porcine submaxillry mucin
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
desialyzed porcine submaxillary mucin
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
glycopeptide + H2O
meconium H-1, H-2 and X-1
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
glycoprotein + H2O
specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
meconium H-1 + H2O
-
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
meconium H-2 + H2O
-
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
meconium X-1 + H2O
low activity
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
stomach + H2O
-
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.51
submaxillary mucin + H2O
desialyzed and not, desialyzed mucine best substrate tested
136753
Bacillus fulminans
alpha-L-fucose + ?
-
136753
Bacillus fulminans
?
3.2.1.63
2'-fucosyllactose + H2O
-
136753
Bacillus fulminans
fucose + lactose
-
-
-
-
3.2.1.63
lacto-N-fucopentaose I
-
136753
Bacillus fulminans
?
-
-
-
-
3.2.1.63
mucin + H2O
porcine submaxillary and glycopeptide of desialyzed mucin
136753
Bacillus fulminans
L-fucose + ?
-
-
-
-
3.2.1.63
O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O
i.e. 2-O-alpha-L-fucosyllactose
136753
Bacillus fulminans
L-fucose + lactose
-
136753
Bacillus fulminans
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
7% loss of activity after 5 min
Bacillus fulminans
3.2.1.51
70
-
77% loss of activity after 5 min
Bacillus fulminans
3.2.1.63
50
-
5 min, no loss of activity
Bacillus fulminans
3.2.1.63
60
-
5 min, 7% loss of activity
Bacillus fulminans
3.2.1.63
70
-
5 min, 77% loss of activity
Bacillus fulminans
3.2.1.63
80
-
5 min, complete loss of activity
Bacillus fulminans