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Literature summary extracted from
- Purification and properties of alpha-L-fucosidase from Bacillus fulminans (1973), J. Biochem., 74, 1141-1149.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.51 | deoxycholate | 34% inhibition at 0.2%, w/v | Bacillus fulminans |  |
| 3.2.1.51 | ethylenediaminetetraacetic acid | 68% inhibition at 0.1 mM | Bacillus fulminans | |
| 3.2.1.51 | Hg2+ | marked inhibition at 0.1 mM | Bacillus fulminans | |
| 3.2.1.51 | Mn2+ | slight inhibition at 0.1 mM | Bacillus fulminans | |
| 3.2.1.51 | SDS | 42% inhibition at 0.02%, w/v | Bacillus fulminans | |
| 3.2.1.63 | deoxycholate | - |
Bacillus fulminans | |
| 3.2.1.63 | EDTA | - |
Bacillus fulminans | |
| 3.2.1.63 | Hg2+ | - |
Bacillus fulminans | |
| 3.2.1.63 | Mn2+ | - |
Bacillus fulminans | |
| 3.2.1.63 | SDS | - |
Bacillus fulminans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.51 | 0.33 | - |
porcine submaxillary mucine, desialyzed | - |
Bacillus fulminans | |
| 3.2.1.51 | 1.6 | - |
glycopeptide from desialyzed porcine submaxillary mucin | - |
Bacillus fulminans | |
| 3.2.1.51 | 2.5 | - |
2'-fucosyllactose | - |
Bacillus fulminans | |
| 3.2.1.63 | 0.175 | - |
glycoprotein | hog, submaxillary | Bacillus fulminans | |
| 3.2.1.63 | 0.33 | - |
porcine submaxillary mucin | - |
Bacillus fulminans | |
| 3.2.1.63 | 1.6 | - |
glycopeptide desialyzed porcine submaxillary mucin | - |
Bacillus fulminans | |
| 3.2.1.63 | 2 | - |
alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4-)-beta-Glc | - |
Bacillus fulminans | |
| 3.2.1.63 | 2.5 | - |
2'-fucosyllactose | - |
Bacillus fulminans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.51 | intracellular | - |
Bacillus fulminans | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.51 | 70000 | 80000 | gel filtration | Bacillus fulminans |
| 3.2.1.63 | 70000 | 80000 | gel filtration | Bacillus fulminans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | Bacillus fulminans | lacto-N-fucopentaose I | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | Bacillus fulminans | 2'-fucosyllactose | alpha-L-fucose + lactose | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O | Bacillus fulminans | - |
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O | - |
? | |
| 3.2.1.51 | glycopeptide + H2O | Bacillus fulminans | desialyzed porcine submaxillary mucin | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | glycopeptide + H2O | Bacillus fulminans | meconium H-1, H-2 and X-1 | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | glycoprotein + H2O | Bacillus fulminans | specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | meconium H-1 + H2O | Bacillus fulminans | - |
alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | meconium H-2 + H2O | Bacillus fulminans | - |
alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | meconium X-1 + H2O | Bacillus fulminans | low activity | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | submaxillary mucin + H2O | Bacillus fulminans | desialyzed and not, desialyzed mucine best substrate tested | alpha-L-fucose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.51 | Bacillus fulminans | - |
- |
- |
| 3.2.1.63 | Bacillus fulminans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.51 | near homogeneity, chromatography techniques | Bacillus fulminans |
| 3.2.1.63 | - |
Bacillus fulminans |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.51 | cell culture | - |
Bacillus fulminans | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.51 | 0.12 | - |
- |
Bacillus fulminans |
| 3.2.1.63 | 0.12 | - |
- |
Bacillus fulminans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaose I | Bacillus fulminans | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | 2'-fucosyllactose | Bacillus fulminans | alpha-L-fucose + lactose | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O | - |
Bacillus fulminans | alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O | - |
? | |
| 3.2.1.51 | glycopeptide + H2O | from meconium H-1, H-2 and X-1 | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | glycopeptide + H2O | desialyzed porcine submaxillry mucin | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | glycopeptide + H2O | desialyzed porcine submaxillary mucin | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | glycopeptide + H2O | meconium H-1, H-2 and X-1 | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | glycopeptide from desialyzed porcine submaxillary mucin + H2O | - |
Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | glycoprotein + H2O | specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | meconium H-1 + H2O | - |
Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | meconium H-2 + H2O | - |
Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | meconium X-1 + H2O | low activity | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | porcine submaxillary mucine, desialyzed + H2O | - |
Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | stomach + H2O | - |
Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | submaxillary mucin + H2O | desialyzed and not, desialyzed mucine best substrate tested | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
| 3.2.1.63 | 2'-fucosyllactose + H2O | - |
Bacillus fulminans | fucose + lactose | - |
? | |
| 3.2.1.63 | alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4-)-beta-Glc + H2O | - |
Bacillus fulminans | alpha-L-fucose + beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glc | - |
? | |
| 3.2.1.63 | glycopeptide desialyzed porcine submaxillary mucin + H2O | - |
Bacillus fulminans | ? | - |
? | |
| 3.2.1.63 | glycoprotein + H2O | - |
Bacillus fulminans | ? | - |
? | |
| 3.2.1.63 | lacto-N-fucopentaose I | - |
Bacillus fulminans | ? | - |
? | |
| 3.2.1.63 | mucin + H2O | porcine submaxillary and glycopeptide of desialyzed mucin | Bacillus fulminans | L-fucose + ? | - |
? | |
| 3.2.1.63 | O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O | i.e. 2-O-alpha-L-fucosyllactose | Bacillus fulminans | L-fucose + lactose | - |
? | |
| 3.2.1.63 | porcine submaxillary mucin + H2O | - |
Bacillus fulminans | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.51 | 50 | - |
7% loss of activity after 5 min | Bacillus fulminans |
| 3.2.1.51 | 70 | - |
77% loss of activity after 5 min | Bacillus fulminans |
| 3.2.1.63 | 50 | - |
5 min, no loss of activity | Bacillus fulminans |
| 3.2.1.63 | 60 | - |
5 min, 7% loss of activity | Bacillus fulminans |
| 3.2.1.63 | 70 | - |
5 min, 77% loss of activity | Bacillus fulminans |
| 3.2.1.63 | 80 | - |
5 min, complete loss of activity | Bacillus fulminans |
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