BRENDA - Enzyme Database

Glycosidases of Aspergillus niger. II. Purification and general properties of 1,2-alpha-L-fucosidase

Bahl, O.P.; J. Biol. Chem. 245, 299-304 (1970)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.63
0.083
-
methyl 2-O-alpha-L-fucopyranosyl-beta-D-galactoside
-
Aspergillus niger
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.63
Aspergillus niger
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.63
-
Aspergillus niger
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.63
additional information
commercial preparation
Aspergillus niger
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.63
6.7
-
-
Aspergillus niger
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.63
2-O-alpha-L-fucopyranosyl-D-galactose + H2O
-
136749
Aspergillus niger
L-fucose + galactose
-
136749
Aspergillus niger
?
3.2.1.63
alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glu + H2O
i.e. lacto-N-fucopentaose I
136749
Aspergillus niger
alpha-L-fucose + beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glu
-
136749
Aspergillus niger
?
3.2.1.63
methyl-2-alpha-L-fucopyranosyl-beta-D-galactoside + H2O
-
136749
Aspergillus niger
L-fucose + methyl beta-D-galactoside
-
136749
Aspergillus niger
?
3.2.1.63
additional information
highly specific for non-reducing terminal L-fucose residues linked to D-galactose residues by 1,2-alpha linkage
136749
Aspergillus niger
?
-
-
-
-
3.2.1.63
mucin + H2O
porcine and canine, submaxillary
136749
Aspergillus niger
L-fucose + ?
-
136749
Aspergillus niger
-
3.2.1.63
O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O
i.e. 2-O-alpha-L-fucosyllactose
136749
Aspergillus niger
L-fucose + lactose
-
136749
Aspergillus niger
?
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.2.1.63
37
-
assay at
Aspergillus niger
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.63
3.8
-
-
Aspergillus niger
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.63
2.4
7.5
-
Aspergillus niger
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.63
0.083
-
methyl 2-O-alpha-L-fucopyranosyl-beta-D-galactoside
-
Aspergillus niger
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.63
-
Aspergillus niger
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.63
additional information
commercial preparation
Aspergillus niger
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.63
6.7
-
-
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.63
2-O-alpha-L-fucopyranosyl-D-galactose + H2O
-
136749
Aspergillus niger
L-fucose + galactose
-
136749
Aspergillus niger
?
3.2.1.63
alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glu + H2O
i.e. lacto-N-fucopentaose I
136749
Aspergillus niger
alpha-L-fucose + beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glu
-
136749
Aspergillus niger
?
3.2.1.63
methyl-2-alpha-L-fucopyranosyl-beta-D-galactoside + H2O
-
136749
Aspergillus niger
L-fucose + methyl beta-D-galactoside
-
136749
Aspergillus niger
?
3.2.1.63
additional information
highly specific for non-reducing terminal L-fucose residues linked to D-galactose residues by 1,2-alpha linkage
136749
Aspergillus niger
?
-
-
-
-
3.2.1.63
mucin + H2O
porcine and canine, submaxillary
136749
Aspergillus niger
L-fucose + ?
-
136749
Aspergillus niger
-
3.2.1.63
O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O
i.e. 2-O-alpha-L-fucosyllactose
136749
Aspergillus niger
L-fucose + lactose
-
136749
Aspergillus niger
?
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.2.1.63
37
-
assay at
Aspergillus niger
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.63
3.8
-
-
Aspergillus niger
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.63
2.4
7.5
-
Aspergillus niger