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Literature summary extracted from

  • Kobayashi, S.; Takaki, Y.; Kobata, K.; Takami, H.; Inoue, A.
    Characterization of maltotetraohydrolase produced Pseudomonas sp. MS300 isolated from the deepest site of the Mariana Trench (1998), Extremophiles, 2, 401-407.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.60 Cu2+ 1 mM CuCl2, amylase A, 98% inhibition, amylase B, complete inhibition Pseudomonas sp.
3.2.1.60 Fe3+ 1 mM FeCl3, amylase A, 73% inhibition, amylase B, 87% inhibition Pseudomonas sp.
3.2.1.60 Hg2+ 1 mM HgCl2, amylase A, 99% inhibition, amylase B, 97% inhibition Pseudomonas sp.
3.2.1.60 Zn2+ 1 mM ZnCl2, amylase A 99% inhibition, amylase B, 97% inhibition Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.60 additional information Pseudomonas sp. more amylase production under high hydrostatic pressure than under atmospheric pressure ?
-
 ?
3.2.1.60 additional information Pseudomonas sp. MS300 more amylase production under high hydrostatic pressure than under atmospheric pressure ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.60 Pseudomonas sp.
-
 MS300
-
3.2.1.60 Pseudomonas sp. MS300
-
 MS300
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.60 amylase A and B Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.60 1544
-
 amylase A Pseudomonas sp.
3.2.1.60 1775
-
 amylase B Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.60 additional information more amylase production under high hydrostatic pressure than under atmospheric pressure Pseudomonas sp. ?
-
 ?
3.2.1.60 additional information more amylase production under high hydrostatic pressure than under atmospheric pressure Pseudomonas sp. MS300 ?
-
 ?
3.2.1.60 starch + H2O amylase A and B potato starch Pseudomonas sp. maltotetraose
-
 ?
3.2.1.60 starch + H2O amylase A and B potato starch Pseudomonas sp. MS300 maltotetraose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.60 50
-
 amylase A and B Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.60 40
-
 amylase A and B, pH 7, full activity below Pseudomonas sp.
3.2.1.60 60
-
 10 min, amylase A and B, inactivation Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.60 6.3
-
 amylase A, 40°C Pseudomonas sp.
3.2.1.60 7.8
-
 amylase B, 40°C Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.60 7 9 amylase B, 40°C, 30 min, stable between Pseudomonas sp.
3.2.1.60 7 8 amylase A, 40°C, 30 min, stable between Pseudomonas sp.