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Literature summary extracted from

  • Nakada, T.; Kubota, M.; Sakai, S.; Tsujisaka, Y.
    Purification and characterization of two forms of maltotetraose-forming amylase from Pseudomonas stutzeri (1990), Agric. Biol. Chem., 54, 737-743.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.60 additional information
-
 additional information
-
 Stutzerimonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.60 46000
-
 G4-2 form, SDS-PAGE Stutzerimonas stutzeri
3.2.1.60 57000
-
 G4-1 form, SDS-PAGE Stutzerimonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.60 Stutzerimonas stutzeri
-
 MO-19
-
3.2.1.60 Stutzerimonas stutzeri MO-19
-
 MO-19
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.60 two active forms, G4-1 and G4-2 Stutzerimonas stutzeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.60 313
-
 G4-1 form Stutzerimonas stutzeri
3.2.1.60 364
-
 G4-2 form Stutzerimonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.60 amylopectin + H2O
-
 Stutzerimonas stutzeri maltotetraose + ?
-
 ?
3.2.1.60 amylopectin + H2O
-
 Stutzerimonas stutzeri MO-19 maltotetraose + ?
-
 ?
3.2.1.60 glycogen + H2O
-
 Stutzerimonas stutzeri maltotetraose
-
 ?
3.2.1.60 glycogen + H2O
-
 Stutzerimonas stutzeri MO-19 maltotetraose
-
 ?
3.2.1.60 starch + H2O
-
 Stutzerimonas stutzeri maltotetraose
-
 ?
3.2.1.60 starch + H2O
-
 Stutzerimonas stutzeri MO-19 maltotetraose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.60 50
-
 G4-1 and G4-2 form Stutzerimonas stutzeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.60 50
-
 G4-1-form heat-sensitive than G4-2 form Stutzerimonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.60 7
-
 G4-1 and G4-2 form Stutzerimonas stutzeri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.60 6.5 9.5 G4-1 and G4-2 form, stable between Stutzerimonas stutzeri