Literature summary extracted from
Zverlov, V.V.; Volkov, I.Y.; Velikodvorskaya, T.V.; Schwarz, W.H.
Highly thermostable endo-1,3-beta-glucanase (laminarinase) LamA from Thermotoga neapolitana: nucleotide sequence of the gen and characterization of the recombinant gene product (1997), Microbiology, 143, 1701-1708.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.2.1.6 |
agriculture |
disease protection of plants because of antimycotic activity in combination with chitinases |
Thermotoga neapolitana |
General Stability
EC Number |
General Stability |
Organism |
---|
3.2.1.6 |
30% of activity at 1% SDS w/v after 20 min incubation |
Thermotoga neapolitana |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.2.1.6 |
additional information |
- |
additional information |
- |
Thermotoga neapolitana |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.6 |
73000 |
- |
SDS-PAGE |
Thermotoga neapolitana |
Organic Solvent Stability
EC Number |
Organic Solvent |
Comment |
Organism |
---|
3.2.1.6 |
Ethanol |
40% of activity after 20 min incubation with 10% v/v ethanol |
Thermotoga neapolitana |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.6 |
Thermotoga neapolitana |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.6 |
from recombinant Escherichia coli cells |
Thermotoga neapolitana |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.6 |
laminarin + H2O |
- |
Thermotoga neapolitana |
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose |
laminaritriose |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.6 |
endo-1,3-beta-glucanase |
- |
Thermotoga neapolitana |
3.2.1.6 |
laminarinase |
- |
Thermotoga neapolitana |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.6 |
85 |
95 |
- |
Thermotoga neapolitana |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.1.6 |
95 |
100 |
incubation at 100°C: 82% of initial activity at 95°C, 57% at 100°C |
Thermotoga neapolitana |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.6 |
6.1 |
6.3 |
- |
Thermotoga neapolitana |