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Literature summary extracted from

  • Meyer, M.T.; Phaff, H.J.
    Purification and properties of (1->3)-alpha-glucanasea from Bacillus circulans WL-12 (1980), J. Gen. Microbiol., 118, 197-208.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.59 EDTA
-
 Niallia circulans

General Stability

EC Number General Stability Organism
3.2.1.59 freezing, -15°C, appreciable loss in activity Niallia circulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.59 Ag+
-
 Niallia circulans
3.2.1.59 Cu2+
-
 Niallia circulans
3.2.1.59 Hg2+
-
 Niallia circulans
3.2.1.59 iodoacetamide
-
 Niallia circulans
3.2.1.59 Mn2+
-
 Niallia circulans
3.2.1.59 Pb2+
-
 Niallia circulans
3.2.1.59 Zn2+
-
 Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.59 additional information
-
 additional information
-
 Niallia circulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.59 extracellular
-
 Niallia circulans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.59 Ca2+ no effect Niallia circulans
3.2.1.59 Co2+ no effect Niallia circulans
3.2.1.59 additional information no requirement of divalent metal Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.59 134000
-
 SDS-PAGE Niallia circulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.59 glucan + H2O Niallia circulans
-
 ?
-
 ?
3.2.1.59 isolichenin + H2O Niallia circulans
-
 ?
-
 ?
3.2.1.59 nigeran + H2O Niallia circulans
-
 ?
-
 ?
3.2.1.59 pseudonigeran + H2O Niallia circulans
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.59 Niallia circulans
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.59 no glycoprotein
-
 Niallia circulans

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.59
-
 Niallia circulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.59 culture filtrate
-
 Niallia circulans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.59 7.52
-
-
 Niallia circulans
3.2.1.59 11.2
-
-
 Niallia circulans

Storage Stability

EC Number Storage Stability Organism
3.2.1.59 4°C, 2 years, pH 6, stable Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.59 glucan + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.59 glucan + H2O alpha-1,3-glucan Niallia circulans nigerose + glucose
-
 ?
3.2.1.59 isolichenin + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.59 additional information
-
 Niallia circulans ?
-
 ?
3.2.1.59 nigeran + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.59 pseudonigeran + H2O
-
 Niallia circulans ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.59 40
-
 5% loss of activity after 90 min Niallia circulans
3.2.1.59 45
-
 25% loss of activity after 90 min Niallia circulans
3.2.1.59 50
-
 complete loss of activity after 20 min Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.59 7.5 8.5
-
 Niallia circulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.59 3.8
-
 50% loss of activity after 10 min Niallia circulans
3.2.1.59 4.2
-
 50% loss of activity after 30 min Niallia circulans
3.2.1.59 5.25 10.5 30°C, stable for at least 1.5 h Niallia circulans