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Literature summary extracted from

  • Pitson, S.M.; Seviour, R.J.; McDougall, B.M.; Woodward, J.R.; Stone, B.A.
    Purification and characterization of three extracellular (1,3)-beta-D-glucan glucohydrolases from the filamentous fungus Acremonium persicinum (1995), Biochem. J., 308, 733-741.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 (NH4)2MoO4
-
 Acremonium persicinum
3.2.1.58 acetamide
-
 Acremonium persicinum
3.2.1.58 BaCl2
-
 Acremonium persicinum
3.2.1.58 CuCl2
-
 Acremonium persicinum
3.2.1.58 FeCl3
-
 Acremonium persicinum
3.2.1.58 HgCl2
-
 Acremonium persicinum
3.2.1.58 iodoacetic acid
-
 Acremonium persicinum
3.2.1.58 KMnO4
-
 Acremonium persicinum
3.2.1.58 MgSO4
-
 Acremonium persicinum
3.2.1.58 MnCl2
-
 Acremonium persicinum
3.2.1.58 N-bromosuccinimide
-
 Acremonium persicinum
3.2.1.58 p-hydroxymercuribenzoate
-
 Acremonium persicinum
3.2.1.58 SnCl2
-
 Acremonium persicinum
3.2.1.58 ZnSO4
-
 Acremonium persicinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.58 0.0018
-
 Laminarin enzyme I, based on molecular mass for laminarin of 5300 Acremonium persicinum
3.2.1.58 0.0041
-
 Laminarin enzyme IIII, based on molecular mass for laminarin of 5300 Acremonium persicinum
3.2.1.58 0.0043
-
 Laminarin enzyme II, based on molecular mass for laminarin of 5300 Acremonium persicinum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.58 extracellular
-
 Acremonium persicinum
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Acremonium persicinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.58 enzyme I 22fold, enzyme II 25fold, enzyme III 28fold Acremonium persicinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.58 culture medium
-
 Acremonium persicinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.58 261
-
 enzyme I Acremonium persicinum
3.2.1.58 292
-
 enzyme II Acremonium persicinum
3.2.1.58 335
-
 enzyme III Acremonium persicinum

Storage Stability

EC Number Storage Stability Organism
3.2.1.58 -20°C, loss of activity in some samples Acremonium persicinum
3.2.1.58 crude and purified enzyme preparations, -80°C, 12 months, no loss of activity Acremonium persicinum
3.2.1.58 crude enzyme preparations, 4°C, 5 weeks, 5% activity loss Acremonium persicinum
3.2.1.58 purified enzyme 4°C, 4 months, no loss of activity Acremonium persicinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 CM-pachyman + H2O
-
 Acremonium persicinum D-glucose
-
 ?
3.2.1.58 curdlan + H2O
-
 Acremonium persicinum ?
-
 ?
3.2.1.58 glucan + H2O Acremonium persicinum extracellular glucan Acremonium persicinum D-glucose + gentiobiose
-
 ?
3.2.1.58 glucan + H2O yeast glucan Acremonium persicinum D-glucose + gentiobiose
-
 ?
3.2.1.58 laminaribiose + H2O
-
 Acremonium persicinum D-glucose
-
 ?
3.2.1.58 laminariheptaose + H2O
-
 Acremonium persicinum ?
-
 ?
3.2.1.58 laminarihexaose + H2O
-
 Acremonium persicinum D-glucose + laminarioligosaccharides
-
 ?
3.2.1.58 laminarin + H2O oxidized laminarin, reduced laminarin Acremonium persicinum D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose ?
3.2.1.58 laminaripentaose + H2O
-
 Acremonium persicinum D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
 ?
3.2.1.58 laminaritetraose + H2O
-
 Acremonium persicinum D-glucose + laminaribiose + laminaritriose
-
 ?
3.2.1.58 laminaritriose + H2O
-
 Acremonium persicinum D-glucose + laminaribiose
-
 ?
3.2.1.58 pachyman + H2O
-
 Acremonium persicinum D-glucose
-
 ?
3.2.1.58 periodate-oxidized and reduced laminarin periodate-oxidized and reduced Acremonium persicinum glycerol + glycoaldehyde + D-glucose
-
 ?
3.2.1.58 schizophyllan + H2O
-
 Acremonium persicinum D-glucose + gentiobiose
-
 ?
3.2.1.58 scleroglucan + H2O
-
 Acremonium persicinum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 81000-89000, SDS-PAGE Acremonium persicinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 55
-
 enzyme II Acremonium persicinum
3.2.1.58 60
-
 enzyme III Acremonium persicinum
3.2.1.58 65
-
 enzyme I Acremonium persicinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.58 50
-
 enzyme II and III, stable below 50°C Acremonium persicinum
3.2.1.58 55
-
 enzyme I, stable below 55°C Acremonium persicinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 5
-
-
 Acremonium persicinum
3.2.1.58 6.5
-
 enzyme I Acremonium persicinum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.58 4.5 7 enzyme II and III Acremonium persicinum
3.2.1.58 5 7.5 enzyme I Acremonium persicinum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.58 4.5 10 enzyme I, II and III Acremonium persicinum