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Literature summary extracted from
- Effect of tunicamycin on exo-1,3-beta-D-glucanase synthesis and secretion by cells and protoplasts of Saccharomyces cerevisiae (1982), J. Gen. Microbiol., 128, 3051-3060.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.58 | 4.07 | - |
p-nitrophenyl-beta-D-glucopyranoside | under glycosylated form | Saccharomyces cerevisiae |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.58 | 43000 | - |
under glycosylated form | Saccharomyces cerevisiae |
| 3.2.1.58 | 58800 | - |
- |
Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.58 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.58 | 4661fold | Saccharomyces cerevisiae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.58 | 13.2 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.58 | 4-methyl-umbelliferyl-beta-D-glucoside | - |
Saccharomyces cerevisiae | 4-methylumbelliferone + D-glucose | - |
? | |
| 3.2.1.58 | laminarin + H2O | - |
Saccharomyces cerevisiae | D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose | different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose | ? | |
| 3.2.1.58 | p-nitrophenyl-beta-D-glucopyranoside | - |
Saccharomyces cerevisiae | p-nitrophenol + D-glucose | - |
? | |
| 3.2.1.58 | pustulan + H2O | - |
Saccharomyces cerevisiae | D-glucose | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.58 | 45 | - |
stable for 45 min, underglycosylated form after 45 min 35% loss of activity | Saccharomyces cerevisiae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.58 | 3.1 | - |
44% loss of activity after 75 min, underglycosylated form 93% loss of activity after 75 min | Saccharomyces cerevisiae |
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