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Literature summary extracted from

  • Iwamuro, Y.; Aoki, M.; Mikami, Y.
    Purification and some properties of an exo-beta-1,3-glucanase from Porodisculus pendulus (1985), J. Ferment. Technol., 63, 405-409.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 Pb2+
-
 Porodisculus pendulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.58 additional information
-
 additional information laminaran 0.080 mg/ml, laminaribiose 4.06 mg/ml , laminarihexaose 0.086 mg/ml, laminaripentaose 0.107 mg/ml, laminaritetraose 0.125 mg/ml, laminaritriose 0.172 mg/ml Porodisculus pendulus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.58 55500
-
 x * 55500, SDS-PAGE Porodisculus pendulus
3.2.1.58 59500
-
 TSK G3000SW, gel filtration Porodisculus pendulus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Porodisculus pendulus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.58 620fold Porodisculus pendulus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.58 27.7
-
-
 Porodisculus pendulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 curdlan + H2O
-
 Porodisculus pendulus ?
-
 ?
3.2.1.58 gentiobiose + H2O
-
 Porodisculus pendulus D-glucose
-
 ?
3.2.1.58 laminaran + H2O
-
 Porodisculus pendulus D-glucose + gentiobiose + gentiotriose almost all D-glucose ?
3.2.1.58 laminaribiose + H2O
-
 Porodisculus pendulus D-glucose
-
 ?
3.2.1.58 laminarihexaose + H2O
-
 Porodisculus pendulus D-glucose + laminarioligosaccharides
-
 ?
3.2.1.58 laminaripentaose + H2O
-
 Porodisculus pendulus D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
 ?
3.2.1.58 laminaritetraose + H2O
-
 Porodisculus pendulus D-glucose + laminaribiose + laminaritriose
-
 ?
3.2.1.58 laminaritriose + H2O
-
 Porodisculus pendulus D-glucose + laminaribiose
-
 ?
3.2.1.58 pendulan + H2O
-
 Porodisculus pendulus D-glucose
-
 ?
3.2.1.58 schizophyllan + H2O
-
 Porodisculus pendulus D-glucose + gentiobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 55500, SDS-PAGE Porodisculus pendulus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 45
-
-
 Porodisculus pendulus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.58 50
-
 stable below 50°C Porodisculus pendulus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 4.5 5
-
 Porodisculus pendulus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.58 5.5 10.5
-
 Porodisculus pendulus