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Literature summary extracted from

  • Marshall, J.J.; Grand, R.J.A.
    Comparative studies on beta-glucan hydrolases. Isolation and characterization of an exo(1 yields 3)-beta-glucanase from the snail, Helix pomatia (1975), Arch. Biochem. Biophys., 167, 165-175.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 AgNO3
-
Helix pomatia
3.2.1.58 Ammonium molybdate
-
Helix pomatia
3.2.1.58 CuSO4
-
Helix pomatia
3.2.1.58 D-glucono-delta-lactone
-
Helix pomatia
3.2.1.58 FeCl2
-
Helix pomatia
3.2.1.58 HgCl2
-
Helix pomatia
3.2.1.58 KCN
-
Helix pomatia
3.2.1.58 N-bromosuccinimide
-
Helix pomatia
3.2.1.58 Phenyl mercuric nitrate
-
Helix pomatia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.58 additional information
-
additional information lichenin 2.22 mg/ml Helix pomatia
3.2.1.58 additional information
-
additional information laminarin 1.22 mg/ml Helix pomatia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.58 82000
-
x * 82000, SDS-PAGE Helix pomatia

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Helix pomatia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.58
-
Helix pomatia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.58 24.2
-
-
Helix pomatia

Storage Stability

EC Number Storage Stability Organism
3.2.1.58 room temperature, several hours Helix pomatia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 cellodextrin + H2O
-
Helix pomatia D-glucose
-
?
3.2.1.58 CM-cellulose + H2O very weak hydrolysis Helix pomatia D-glucose
-
?
3.2.1.58 gentiobiose + H2O
-
Helix pomatia D-glucose
-
?
3.2.1.58 glucan + H2O barley beta-glucan Helix pomatia D-glucose + gentiobiose D-glucose only ?
3.2.1.58 glucan + H2O yeast glucan Helix pomatia D-glucose + gentiobiose D-glucose only ?
3.2.1.58 glucan + H2O oat beta-glucan Helix pomatia D-glucose + gentiobiose D-glucose only ?
3.2.1.58 laminaribiose + H2O
-
Helix pomatia D-glucose
-
?
3.2.1.58 laminarin + H2O
-
Helix pomatia D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose ir
3.2.1.58 laminaripentaose + H2O
-
Helix pomatia D-glucose + laminaribiose + laminaritriose + laminaritetraose different combinations and amount of these products, depending on organism ?
3.2.1.58 laminaritetraose + H2O
-
Helix pomatia D-glucose + laminaribiose + laminaritriose different combinations and amount of these products, depending on organism ?
3.2.1.58 laminaritriose + H2O
-
Helix pomatia D-glucose + laminaribiose D-glucose only ?
3.2.1.58 lichenin + H2O
-
Helix pomatia D-glucose
-
?
3.2.1.58 luteose + H2O
-
Helix pomatia D-glucose
-
?
3.2.1.58 pachyman + H2O
-
Helix pomatia D-glucose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 82000, SDS-PAGE Helix pomatia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 53
-
-
Helix pomatia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.58 50
-
up to 50°C Helix pomatia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 4.4 4.5
-
Helix pomatia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.58 3 6.5
-
Helix pomatia