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Literature summary extracted from
- Two components of cell-bound isopullulanase from Aspergillus niger ATCC 9642 - Their purification and enzymatic properties (1996), Biosci. Biotechnol. Biochem., 60, 1795-1798.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.57 | Fe3+ | - |
Aspergillus niger |  |
| 3.2.1.57 | N-bromosuccinimide | - |
Aspergillus niger | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.57 | cell bound | - |
Aspergillus niger | - |
- |
| 3.2.1.57 | extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.57 | 59000 | - |
F1 and F2 deglycosylated | Aspergillus niger |
| 3.2.1.57 | 69000 | - |
F2, SDS-PAGE | Aspergillus niger |
| 3.2.1.57 | 71000 | - |
F1, SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.57 | pullulan + H2O | Aspergillus niger | - |
isopanose + (Glc)4 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.57 | Aspergillus niger | - |
ATCC 9642, IPU F1 and IPU F2 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.57 | glycoprotein | - |
Aspergillus niger |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.57 | - |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.57 | 27 | - |
F2 | Aspergillus niger |
| 3.2.1.57 | 27.3 | - |
F1 | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.57 | alpha-maltosylmaltose + H2O | - |
Aspergillus niger | isopanose + glucose | - |
? | |
| 3.2.1.57 | panose + H2O | - |
Aspergillus niger | isomaltose + glucose | - |
? | |
| 3.2.1.57 | pullulan + H2O | - |
Aspergillus niger | isopanose + (Glc)4 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.57 | 40 | - |
F2 at pH 3.5 | Aspergillus niger |
| 3.2.1.57 | 45 | - |
F1 at pH 3.5 | Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.57 | 35 | - |
F2, below, 30 min at pH 3.5 | Aspergillus niger |
| 3.2.1.57 | 45 | - |
F1, below, 30 min at pH 3.5 | Aspergillus niger |
| 3.2.1.57 | 55 | - |
F2, loss of activity | Aspergillus niger |
| 3.2.1.57 | 60 | - |
F1, loss of activity | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.57 | 3 | 4 | - |
Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.57 | 2 | 7 | at 4°C 12 h | Aspergillus niger |
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