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Literature summary extracted from

  • Yang, S.J.; Wang, Z.; Zhang, S.Z.
    Purification and properties of cyclodextrinase from Bacillus stearothermophilus HY-1 (1996), Ann. N. Y. Acad. Sci., 799, 425-428.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.54 Cu2+ relative activity 1.6% Geobacillus stearothermophilus
3.2.1.54 diethyldicarbonate DEPC 10 mM, relative activity 2.3% Geobacillus stearothermophilus
3.2.1.54 Fe3+ relative activity 33% Geobacillus stearothermophilus
3.2.1.54 Hg2+ relative activity 1.6% Geobacillus stearothermophilus
3.2.1.54 N-bromosuccinimide 0.1 mM, relative activity 8%, 1.0 mM, relative activity 4.6% Geobacillus stearothermophilus
3.2.1.54 Zn2+ relative activity 1.5% Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 61000
-
 SDS-PAGE Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Geobacillus stearothermophilus
-
 HY-1
-
3.2.1.54 Geobacillus stearothermophilus HY-1
-
 HY-1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 amylopectin + H2O
-
 Geobacillus stearothermophilus maltose + ?
-
 ?
3.2.1.54 amylopectin + H2O
-
 Geobacillus stearothermophilus HY-1 maltose + ?
-
 ?
3.2.1.54 amylose + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
3.2.1.54 amylose + H2O
-
 Geobacillus stearothermophilus HY-1 ?
-
 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 Geobacillus stearothermophilus maltodextrin
-
 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 Geobacillus stearothermophilus HY-1 maltodextrin
-
 ?
3.2.1.54 pullulan + H2O
-
 Geobacillus stearothermophilus panose + maltose + glucose
-
 ?
3.2.1.54 pullulan + H2O
-
 Geobacillus stearothermophilus HY-1 panose + maltose + glucose
-
 ?
3.2.1.54 starch + H2O
-
 Geobacillus stearothermophilus glucose + maltose
-
 ?
3.2.1.54 starch + H2O
-
 Geobacillus stearothermophilus HY-1 glucose + maltose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 55
-
-
 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 6.2
-
-
 Geobacillus stearothermophilus