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Literature summary extracted from

  • Kim, M.J.; Park, W.S.; Lee, H.S.; Kim, T.J.; Shin, J.H.; Yoo, S.H.; Cheong, T.K.; Ryu, S.; Kim, J.C.; Kim, K.W.; Moon, T.W.; Robyt, J.F.; Park, K.H.
    Kinetics and inhibition of cyclomaltodextrinase from alkalophilic Bacillus sp. I-5 (2000), Arch. Biochem. Biophys., 373, 110-115.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 CDase I-5 gene, pBR322, expression in Escherichia coli MC1061 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 0.83
-
beta-cyclodextrin
-
Bacillus sp. (in: Bacteria)
3.2.1.54 0.92
-
gamma-cyclodextrin
-
Bacillus sp. (in: Bacteria)
3.2.1.54 1.23
-
alpha-cyclodextrin
-
Bacillus sp. (in: Bacteria)
3.2.1.54 2.11
-
pullulan
-
Bacillus sp. (in: Bacteria)
3.2.1.54 3.01
-
soluble starch
-
Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 63000
-
MW deduced from the nucleotide sequence 64884 Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Bacillus sp. (in: Bacteria)
-
I-5
-
3.2.1.54 Bacillus sp. (in: Bacteria) I-5
-
I-5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 acarbose + H2O pseudotetrasaccharide inhibitor of glucosidases and alpha-amylases, binding the active sites of the enzyme Bacillus sp. (in: Bacteria) ?
-
?
3.2.1.54 acarbose + H2O pseudotetrasaccharide inhibitor of glucosidases and alpha-amylases, binding the active sites of the enzyme Bacillus sp. (in: Bacteria) I-5 ?
-
?
3.2.1.54 alpha-cyclomaltodextrin + H2O
-
Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
?
3.2.1.54 alpha-cyclomaltodextrin + H2O
-
Bacillus sp. (in: Bacteria) I-5 alpha-D-glucose + maltose
-
?
3.2.1.54 beta-cyclodextrin + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
3.2.1.54 beta-cyclodextrin + H2O
-
Bacillus sp. (in: Bacteria) I-5 ?
-
?
3.2.1.54 soluble starch + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
3.2.1.54 soluble starch + H2O
-
Bacillus sp. (in: Bacteria) I-5 ?
-
?