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Literature summary extracted from
- Purification and properties of cyclodextrinase from Bacillus stearothermophilus HY-1 (1996), Appl. Biochem. Biotechnol., 59, 63-75.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.54 | Cu2+ | - |
Geobacillus stearothermophilus |  |
| 3.2.1.54 | Fe3+ | slight inhibition | Geobacillus stearothermophilus | |
| 3.2.1.54 | Hg2+ | - |
Geobacillus stearothermophilus | |
| 3.2.1.54 | Pb2+ | - |
Geobacillus stearothermophilus | |
| 3.2.1.54 | Zn2+ | - |
Geobacillus stearothermophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.54 | 1.67 | - |
beta-cyclodextrin | - |
Geobacillus stearothermophilus | |
| 3.2.1.54 | 1.79 | - |
alpha-cyclodextrin | - |
Geobacillus stearothermophilus | |
| 3.2.1.54 | 2.5 | - |
gamma-cyclodextrin | - |
Geobacillus stearothermophilus | |
| 3.2.1.54 | 8.3 | - |
pullulan | - |
Geobacillus stearothermophilus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.54 | Mg2+ | increase activity to 112%/102% | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.54 | 61000 | - |
- |
Geobacillus stearothermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.54 | Geobacillus stearothermophilus | - |
HY-1 | - |
| 3.2.1.54 | Geobacillus stearothermophilus HY-1 | - |
HY-1 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.54 | purified 230fold | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.54 | 33.4 | - |
affinity chromatography | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.54 | alpha-cyclodextrin | - |
Geobacillus stearothermophilus | maltotriose + maltose + glucose | - |
? | |
| 3.2.1.54 | alpha-cyclodextrin | - |
Geobacillus stearothermophilus HY-1 | maltotriose + maltose + glucose | - |
? | |
| 3.2.1.54 | amylopectin + H2O | - |
Geobacillus stearothermophilus | maltose + ? | - |
? | |
| 3.2.1.54 | amylose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
| 3.2.1.54 | amylose + H2O | - |
Geobacillus stearothermophilus HY-1 | ? | - |
? | |
| 3.2.1.54 | beta-cyclodextrin + H2O | - |
Geobacillus stearothermophilus | maltose + D-glucose | - |
? | |
| 3.2.1.54 | cyclomaltodextrin + H2O | - |
Geobacillus stearothermophilus | maltodextrin | - |
? | |
| 3.2.1.54 | cyclomaltodextrin + H2O | - |
Geobacillus stearothermophilus HY-1 | maltodextrin | - |
? | |
| 3.2.1.54 | gamma-cyclodextrin + H2O | - |
Geobacillus stearothermophilus | maltose + D-glucose | - |
? | |
| 3.2.1.54 | maltodextrins + H2O | linear, enzyme degrades the linear molecules by splitting off maltose units from the nonreducing end of the chain | Geobacillus stearothermophilus | maltose + glucose | - |
? | |
| 3.2.1.54 | maltodextrins + H2O | linear, enzyme degrades the linear molecules by splitting off maltose units from the nonreducing end of the chain | Geobacillus stearothermophilus HY-1 | maltose + glucose | - |
? | |
| 3.2.1.54 | pullulan + H2O | - |
Geobacillus stearothermophilus | panose + maltose + glucose | - |
? | |
| 3.2.1.54 | starch + H2O | - |
Geobacillus stearothermophilus | glucose + maltose | - |
? | |
| 3.2.1.54 | starch + H2O | - |
Geobacillus stearothermophilus HY-1 | glucose + maltose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.54 | 55 | - |
- |
Geobacillus stearothermophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.54 | 40 | 70 | - |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.54 | 50 | - |
stable at temperatures below | Geobacillus stearothermophilus |
| 3.2.1.54 | 60 | - |
- |
Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.54 | 6.2 | - |
- |
Geobacillus stearothermophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.54 | 5 | 7.5 | - |
Geobacillus stearothermophilus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.54 | 5.8 | 7.6 | - |
Geobacillus stearothermophilus |
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