Purification and properties of the cyclodextrinase of Bacillus sphaericus ATCC 7055
Galvin, N.M.; Kelly, C.T.; Fogarty, W.M.; Appl. Microbiol. Biotechnol. 42, 46-50 (1994)
No PubMed abstract available
Data extracted from this reference:
Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.54
2-deoxy-D-glucose
0.01 M, relative activity 140%
Lysinibacillus sphaericus
3.2.1.54
glucosamine
0.01 M, relative activity 133%
Lysinibacillus sphaericus
3.2.1.54
glucose
0.01 M, relative activity 143%
Lysinibacillus sphaericus
3.2.1.54
glucose 6-phosphate
0.005 M, relative activity 111%
Lysinibacillus sphaericus
3.2.1.54
maltose
0.01 M, relative activity 140%
Lysinibacillus sphaericus
3.2.1.54
miglitol
1 mg/ml, relative activity 107%
Lysinibacillus sphaericus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.54
acarbose
-
Lysinibacillus sphaericus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.54
3.9
-
beta-cyclodextrin
-
Lysinibacillus sphaericus
3.2.1.54
4
-
alpha-cyclodextrin
-
Lysinibacillus sphaericus
3.2.1.54
4.2
-
gamma-cyclodextrin
-
Lysinibacillus sphaericus
3.2.1.54
5.8
-
maltoheptaose
-
Lysinibacillus sphaericus
3.2.1.54
6.1
-
maltohexaose
-
Lysinibacillus sphaericus
3.2.1.54
7.5
-
maltotetraose
-
Lysinibacillus sphaericus
3.2.1.54
14
-
maltotriose
-
Lysinibacillus sphaericus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.54
91200
95000
gel filtration
Lysinibacillus sphaericus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.54
Lysinibacillus sphaericus
-
ATCC 7055
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.54
-
Lysinibacillus sphaericus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.54
366.3
-
homogenous by SDS-PAGE
Lysinibacillus sphaericus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.54
alpha-cyclomaltodextrin + H2O
-
136620
Lysinibacillus sphaericus
alpha-D-glucose + maltose
-
-
-
?
3.2.1.54
beta-cyclodextrin + H2O
-
136620
Lysinibacillus sphaericus
maltose + glucose
-
-
-
?
3.2.1.54
beta-cyclodextrin + H2O
-
136620
Lysinibacillus sphaericus
glucose + maltose + maltotriose + maltotetraose
-
136620
Lysinibacillus sphaericus
?
3.2.1.54
branched cyclodextrins + H2O
-
136620
Lysinibacillus sphaericus
glucose + maltose
-
136620
Lysinibacillus sphaericus
?
3.2.1.54
cyclomaltodextrin + H2O
-
136620
Lysinibacillus sphaericus
maltodextrin
-
-
-
?
3.2.1.54
gamma-cyclodextrin + H2O
-
136620
Lysinibacillus sphaericus
glucose + maltose + maltotriose + maltotetraose + maltopentaose + maltohexaose + maltoheptaose + maltooctaose
-
-
-
?
3.2.1.54
glucosyl-beta-cyclodextrin + H2O
-
136620
Lysinibacillus sphaericus
6,4-O-alpha-D-glucosylmaltoheptaose
-
-
-
?
3.2.1.54
maltoheptaose + H2O
-
136620
Lysinibacillus sphaericus
D-glucose + maltose
-
136620
Lysinibacillus sphaericus
?
3.2.1.54
maltopentaose + H2O
-
136620
Lysinibacillus sphaericus
maltose + maltotriose
-
-
-
?
3.2.1.54
maltosyl-alpha-cyclodextrin + H2O
-
136620
Lysinibacillus sphaericus
6,3-O-alpha-D-maltosylmaltohexaose
-
-
-
?
3.2.1.54
maltosyl-beta-cyclodextrin
-
136620
Lysinibacillus sphaericus
6,4-O-alpha-D-maltosylmaltoheptaose
-
-
-
?
3.2.1.54
pullulan + H2O
-
136620
Lysinibacillus sphaericus
glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds
-
-
-
?
3.2.1.54
starch + H2O
-
136620
Lysinibacillus sphaericus
glucose + maltose
-
136620
Lysinibacillus sphaericus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.54
40
-
-
Lysinibacillus sphaericus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.54
40
-
100% stable for 30 min up to 40°C
Lysinibacillus sphaericus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.54
6
6.5
97% activity at pH 7
Lysinibacillus sphaericus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.54
6
7
100% stable
Lysinibacillus sphaericus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.54
2-deoxy-D-glucose
0.01 M, relative activity 140%
Lysinibacillus sphaericus
3.2.1.54
glucosamine
0.01 M, relative activity 133%
Lysinibacillus sphaericus
3.2.1.54
glucose
0.01 M, relative activity 143%
Lysinibacillus sphaericus
3.2.1.54
glucose 6-phosphate
0.005 M, relative activity 111%
Lysinibacillus sphaericus
3.2.1.54
maltose
0.01 M, relative activity 140%
Lysinibacillus sphaericus
3.2.1.54
miglitol
1 mg/ml, relative activity 107%
Lysinibacillus sphaericus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.54
acarbose
-
Lysinibacillus sphaericus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.54
3.9
-
beta-cyclodextrin
-
Lysinibacillus sphaericus
3.2.1.54
4
-
alpha-cyclodextrin
-
Lysinibacillus sphaericus
3.2.1.54
4.2
-
gamma-cyclodextrin
-
Lysinibacillus sphaericus
3.2.1.54
5.8
-
maltoheptaose
-
Lysinibacillus sphaericus
3.2.1.54
6.1
-
maltohexaose
-
Lysinibacillus sphaericus
3.2.1.54
7.5
-
maltotetraose
-
Lysinibacillus sphaericus
3.2.1.54
14
-
maltotriose
-
Lysinibacillus sphaericus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.54
91200
95000
gel filtration
Lysinibacillus sphaericus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.54
-
Lysinibacillus sphaericus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.54
366.3
-
homogenous by SDS-PAGE
Lysinibacillus sphaericus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.54
alpha-cyclomaltodextrin + H2O
-
136620
Lysinibacillus sphaericus
alpha-D-glucose + maltose
-
-
-
?
3.2.1.54
beta-cyclodextrin + H2O
-
136620
Lysinibacillus sphaericus
maltose + glucose
-
-
-
?
3.2.1.54
beta-cyclodextrin + H2O
-
136620
Lysinibacillus sphaericus
glucose + maltose + maltotriose + maltotetraose
-
136620
Lysinibacillus sphaericus
?
3.2.1.54
branched cyclodextrins + H2O
-
136620
Lysinibacillus sphaericus
glucose + maltose
-
136620
Lysinibacillus sphaericus
?
3.2.1.54
cyclomaltodextrin + H2O
-
136620
Lysinibacillus sphaericus
maltodextrin
-
-
-
?
3.2.1.54
gamma-cyclodextrin + H2O
-
136620
Lysinibacillus sphaericus
glucose + maltose + maltotriose + maltotetraose + maltopentaose + maltohexaose + maltoheptaose + maltooctaose
-
-
-
?
3.2.1.54
glucosyl-beta-cyclodextrin + H2O
-
136620
Lysinibacillus sphaericus
6,4-O-alpha-D-glucosylmaltoheptaose
-
-
-
?
3.2.1.54
maltoheptaose + H2O
-
136620
Lysinibacillus sphaericus
D-glucose + maltose
-
136620
Lysinibacillus sphaericus
?
3.2.1.54
maltopentaose + H2O
-
136620
Lysinibacillus sphaericus
maltose + maltotriose
-
-
-
?
3.2.1.54
maltosyl-alpha-cyclodextrin + H2O
-
136620
Lysinibacillus sphaericus
6,3-O-alpha-D-maltosylmaltohexaose
-
-
-
?
3.2.1.54
maltosyl-beta-cyclodextrin
-
136620
Lysinibacillus sphaericus
6,4-O-alpha-D-maltosylmaltoheptaose
-
-
-
?
3.2.1.54
pullulan + H2O
-
136620
Lysinibacillus sphaericus
glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds
-
-
-
?
3.2.1.54
starch + H2O
-
136620
Lysinibacillus sphaericus
glucose + maltose
-
136620
Lysinibacillus sphaericus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.54
40
-
-
Lysinibacillus sphaericus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.54
40
-
100% stable for 30 min up to 40°C
Lysinibacillus sphaericus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.54
6
6.5
97% activity at pH 7
Lysinibacillus sphaericus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.54
6
7
100% stable
Lysinibacillus sphaericus