EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.54 | 2-deoxy-D-glucose | 0.01 M, relative activity 140% | Lysinibacillus sphaericus | |
3.2.1.54 | glucosamine | 0.01 M, relative activity 133% | Lysinibacillus sphaericus | |
3.2.1.54 | glucose | 0.01 M, relative activity 143% | Lysinibacillus sphaericus | |
3.2.1.54 | glucose 6-phosphate | 0.005 M, relative activity 111% | Lysinibacillus sphaericus | |
3.2.1.54 | maltose | 0.01 M, relative activity 140% | Lysinibacillus sphaericus | |
3.2.1.54 | miglitol | 1 mg/ml, relative activity 107% | Lysinibacillus sphaericus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.54 | acarbose | - |
Lysinibacillus sphaericus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.54 | 3.9 | - |
beta-cyclodextrin | - |
Lysinibacillus sphaericus | |
3.2.1.54 | 4 | - |
alpha-cyclodextrin | - |
Lysinibacillus sphaericus | |
3.2.1.54 | 4.2 | - |
gamma-cyclodextrin | - |
Lysinibacillus sphaericus | |
3.2.1.54 | 5.8 | - |
maltoheptaose | - |
Lysinibacillus sphaericus | |
3.2.1.54 | 6.1 | - |
maltohexaose | - |
Lysinibacillus sphaericus | |
3.2.1.54 | 7.5 | - |
maltotetraose | - |
Lysinibacillus sphaericus | |
3.2.1.54 | 14 | - |
maltotriose | - |
Lysinibacillus sphaericus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 91200 | 95000 | gel filtration | Lysinibacillus sphaericus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.54 | Lysinibacillus sphaericus | - |
ATCC 7055 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.54 | - |
Lysinibacillus sphaericus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 366.3 | - |
homogenous by SDS-PAGE | Lysinibacillus sphaericus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.54 | alpha-cyclomaltodextrin + H2O | - |
Lysinibacillus sphaericus | alpha-D-glucose + maltose | - |
? | |
3.2.1.54 | beta-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | maltose + glucose | - |
? | |
3.2.1.54 | beta-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | glucose + maltose + maltotriose + maltotetraose | - |
? | |
3.2.1.54 | branched cyclodextrins + H2O | - |
Lysinibacillus sphaericus | glucose + maltose | - |
? | |
3.2.1.54 | cyclomaltodextrin + H2O | - |
Lysinibacillus sphaericus | maltodextrin | - |
? | |
3.2.1.54 | gamma-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | glucose + maltose + maltotriose + maltotetraose + maltopentaose + maltohexaose + maltoheptaose + maltooctaose | - |
? | |
3.2.1.54 | glucosyl-beta-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | 6,4-O-alpha-D-glucosylmaltoheptaose | - |
? | |
3.2.1.54 | maltoheptaose + H2O | - |
Lysinibacillus sphaericus | D-glucose + maltose + ? | - |
? | |
3.2.1.54 | maltopentaose + H2O | - |
Lysinibacillus sphaericus | maltose + maltotriose | - |
? | |
3.2.1.54 | maltosyl-alpha-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | 6,3-O-alpha-D-maltosylmaltohexaose | - |
? | |
3.2.1.54 | maltosyl-beta-cyclodextrin | - |
Lysinibacillus sphaericus | 6,4-O-alpha-D-maltosylmaltoheptaose | - |
? | |
3.2.1.54 | pullulan + H2O | - |
Lysinibacillus sphaericus | glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds | - |
? | |
3.2.1.54 | starch + H2O | - |
Lysinibacillus sphaericus | glucose + maltose | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 40 | - |
- |
Lysinibacillus sphaericus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 40 | - |
100% stable for 30 min up to 40°C | Lysinibacillus sphaericus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 6 | 6.5 | 97% activity at pH 7 | Lysinibacillus sphaericus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 6 | 7 | 100% stable | Lysinibacillus sphaericus |