Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Podkovyrov, S.M.; Zeikus, J.G.
    Structure of the gene encoding cyclomaltodextrinase from Clostridium thermohydrosulfuricum 39E and characterization of the enzyme purified from Escherichia coli (1992), J. Bacteriol., 8, 5400-5405.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 plasmid pPG22, expression in Escherichia coli Thermoanaerobacter thermohydrosulfuricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.54 Ag+
-
 Thermoanaerobacter thermohydrosulfuricus
3.2.1.54 Cu2+
-
 Thermoanaerobacter thermohydrosulfuricus
3.2.1.54 Hg2+
-
 Thermoanaerobacter thermohydrosulfuricus
3.2.1.54 N-bromosuccinimide
-
 Thermoanaerobacter thermohydrosulfuricus
3.2.1.54 p-chloromercuribenzoate
-
 Thermoanaerobacter thermohydrosulfuricus
3.2.1.54 Zn2+
-
 Thermoanaerobacter thermohydrosulfuricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 1.3
-
 gamma-cyclodextrin
-
 Thermoanaerobacter thermohydrosulfuricus
3.2.1.54 2.1
-
 beta-cyclodextrin
-
 Thermoanaerobacter thermohydrosulfuricus
3.2.1.54 2.5
-
 alpha-cyclodextrin
-
 Thermoanaerobacter thermohydrosulfuricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 66000
-
 monomer SDS-PAGE Thermoanaerobacter thermohydrosulfuricus
3.2.1.54 68030
-
 denatured protein estimated by SDS-PAGE, native enzyme determined by gel filtration Thermoanaerobacter thermohydrosulfuricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Thermoanaerobacter thermohydrosulfuricus
-
 39E
-
3.2.1.54 Thermoanaerobacter thermohydrosulfuricus 39E
-
 39E
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 gene encoding enzyme cloned in Escherichia coli, expressed and purified 12fold from Escherichia coli Thermoanaerobacter thermohydrosulfuricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 62
-
 gel filtration Thermoanaerobacter thermohydrosulfuricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclodextrin + H2O
-
 Thermoanaerobacter thermohydrosulfuricus maltose + D-glucose
-
 ?
3.2.1.54 amylopectin + H2O rate of hydrolysis less than 5% of the rate of alpha-cyclodextrin hydrolysis Thermoanaerobacter thermohydrosulfuricus maltose + ?
-
 ?
3.2.1.54 amylose + H2O less than 5% of the rate of alpha-cyclodextrin hydrolysis Thermoanaerobacter thermohydrosulfuricus ?
-
 ?
3.2.1.54 beta-cyclodextrin + H2O
-
 Thermoanaerobacter thermohydrosulfuricus glucose + maltose + long chain linear oligomers
-
 ?
3.2.1.54 beta-cyclodextrin + H2O
-
 Thermoanaerobacter thermohydrosulfuricus 39E glucose + maltose + long chain linear oligomers
-
 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 Thermoanaerobacter thermohydrosulfuricus maltodextrin
-
 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 Thermoanaerobacter thermohydrosulfuricus 39E maltodextrin
-
 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
 Thermoanaerobacter thermohydrosulfuricus maltose + D-glucose
-
 ?
3.2.1.54 glycogen + H2O relative rate of hydrolysis 0,1% Thermoanaerobacter thermohydrosulfuricus ?
-
 ?
3.2.1.54 maltodextrins + H2O linear, enzyme degrades the linear molecules by splitting off maltose units from the nonreducing end of the chain Thermoanaerobacter thermohydrosulfuricus maltose + glucose
-
 ?
3.2.1.54 maltodextrins + H2O linear, enzyme degrades the linear molecules by splitting off maltose units from the nonreducing end of the chain Thermoanaerobacter thermohydrosulfuricus 39E maltose + glucose
-
 ?
3.2.1.54 maltoheptaose + H2O
-
 Thermoanaerobacter thermohydrosulfuricus D-glucose + maltose + ?
-
 ?
3.2.1.54 maltohexaose + H2O
-
 Thermoanaerobacter thermohydrosulfuricus maltotetraose + maltose
-
 ?
3.2.1.54 maltopentaose + H2O
-
 Thermoanaerobacter thermohydrosulfuricus maltose + maltotriose
-
 ?
3.2.1.54 maltotetraose + H2O
-
 Thermoanaerobacter thermohydrosulfuricus 2 maltose
-
 ?
3.2.1.54 maltotriose + H2O slowly Thermoanaerobacter thermohydrosulfuricus ?
-
 ?
3.2.1.54 maltotriose + H2O slowly Thermoanaerobacter thermohydrosulfuricus 39E ?
-
 ?
3.2.1.54 additional information not: maltose Thermoanaerobacter thermohydrosulfuricus ?
-
 ?
3.2.1.54 additional information not: maltose Thermoanaerobacter thermohydrosulfuricus 39E ?
-
 ?
3.2.1.54 pullulan + H2O rate of hydrolysis less than 5% of the rate of alpha-cyclodextrin hydrolysis Thermoanaerobacter thermohydrosulfuricus glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds
-
 ?
3.2.1.54 starch + H2O
-
 Thermoanaerobacter thermohydrosulfuricus glucose + maltose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 thermophilic CDase
-
 Thermoanaerobacter thermohydrosulfuricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 65
-
 beta-cyclodextrin as substrate Thermoanaerobacter thermohydrosulfuricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.54 50 75
-
 Thermoanaerobacter thermohydrosulfuricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.54 65
-
 incubation for 1 h led to a 15% loss of activity Thermoanaerobacter thermohydrosulfuricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 5.9
-
-
 Thermoanaerobacter thermohydrosulfuricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 4 10
-
 Thermoanaerobacter thermohydrosulfuricus