Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • DePinto, J.A.; Campbell, L.L.
    Purification and properties of the cyclodextrinase of Bacillus macerans (1968), Biochemistry, 7, 121-125.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 2.62
-
 alpha-cyclodextrin
-
 Paenibacillus macerans
3.2.1.54 2.65
-
 beta-cyclodextrin
-
 Paenibacillus macerans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.54 cyclomaltodextrins + H2O Paenibacillus macerans
-
 linear maltodextrins
-
 ?
3.2.1.54 cyclomaltodextrins + H2O Paenibacillus macerans ATCC 8514
-
 linear maltodextrins
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Paenibacillus macerans
-
 ATCC8514
-
3.2.1.54 Paenibacillus macerans ATCC 8514
-
 ATCC8514
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 purified 40fold, highly purified, not completely free from contaminations Paenibacillus macerans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 additional information
-
-
 Paenibacillus macerans
3.2.1.54 5
-
 crude enzyme, purified 1fold Paenibacillus macerans
3.2.1.54 175
-
-
 Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclomaltodextrin + H2O
-
 Paenibacillus macerans alpha-D-glucose + maltose
-
 ?
3.2.1.54 alpha-cyclomaltodextrin + H2O
-
 Paenibacillus macerans ATCC 8514 alpha-D-glucose + maltose
-
 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 Paenibacillus macerans maltodextrin
-
 ir
3.2.1.54 cyclomaltodextrin + H2O
-
 Paenibacillus macerans ATCC 8514 maltodextrin
-
 ir
3.2.1.54 cyclomaltodextrins + H2O
-
 Paenibacillus macerans linear maltodextrins
-
 ?
3.2.1.54 cyclomaltodextrins + H2O
-
 Paenibacillus macerans ATCC 8514 linear maltodextrins
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.54 30 55
-
 Paenibacillus macerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 6.2 6.4
-
 Paenibacillus macerans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 6 7.6
-
 Paenibacillus macerans