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Literature summary extracted from

  • Kitahata, S.; Taniguchi, M.; Beltran, S.D.; Sugimoto, T.; Okada, S.
    Purification and some properties of cyclodextrinase from Bacillus coagulans (1983), Agric. Biol. Chem., 47, 1441-1447.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.54 Fe2+ reduces activity to79% Weizmannia coagulans
3.2.1.54 Fe3+ reduces activity to 50% Weizmannia coagulans
3.2.1.54 Hg2+ reduces activity to 24% Weizmannia coagulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 0.47
-
 gamma-cyclodextrin
-
 Weizmannia coagulans
3.2.1.54 1.4
-
 maltohexaose
-
 Weizmannia coagulans
3.2.1.54 2.3
-
 maltopentaose
-
 Weizmannia coagulans
3.2.1.54 2.8
-
 beta-cyclodextrin
-
 Weizmannia coagulans
3.2.1.54 4
-
 maltotetraose
-
 Weizmannia coagulans
3.2.1.54 4.5
-
 maltotriose
-
 Weizmannia coagulans
3.2.1.54 10
-
 alpha-cyclodextrin
-
 Weizmannia coagulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.54 intracellular
-
 Weizmannia coagulans 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.54 Mg2+ increase activity to 112%/102% Weizmannia coagulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 62000
-
 SDS-PAGE Weizmannia coagulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.54 cyclomaltodextrins + H2O Weizmannia coagulans
-
 linear maltodextrins
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Weizmannia coagulans
-
 gram positive bacterium, isolated from soil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 not completely purified, minor contamination in ultracentrifugation Weizmannia coagulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 additional information
-
-
 Weizmannia coagulans
3.2.1.54 0.12
-
 crude enzyme Weizmannia coagulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 cyclomaltodextrin + H2O alpha-, beta- and gamma Weizmannia coagulans maltodextrin
-
 ?
3.2.1.54 cyclomaltodextrins + H2O
-
 Weizmannia coagulans linear maltodextrins
-
 ?
3.2.1.54 maltodextrins + H2O
-
 Weizmannia coagulans maltose + glucose
-
 ?
3.2.1.54 maltohexaose + H2O
-
 Weizmannia coagulans maltotetraose + maltose
-
 ?
3.2.1.54 maltopentaose + H2O
-
 Weizmannia coagulans maltose + maltotriose
-
 ?
3.2.1.54 maltotetraose + H2O
-
 Weizmannia coagulans 2 maltose
-
 ?
3.2.1.54 maltotriose + H2O slowly Weizmannia coagulans ?
-
 ?
3.2.1.54 additional information slowly: short chain amylose Weizmannia coagulans ?
-
 ?
3.2.1.54 additional information not: maltose Weizmannia coagulans ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 50
-
-
 Weizmannia coagulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.54 40
-
 stable up at pH 6.0-7.3 on 2 h incubation Weizmannia coagulans
3.2.1.54 45
-
 stable at temperatures below , pH 7 Weizmannia coagulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 6.2
-
-
 Weizmannia coagulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 5 8
-
 Weizmannia coagulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.54 6 7.3 40°C, 2 h stable Weizmannia coagulans